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The picture on the left shows the staining obtaoned, at low power magnification. It shows the pattern of staining in the cortex and hippocampus (Hip.). Right: Using higher power magnification at the level of the hippocampus.
The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
Dr. Sophie Pezet
Submitted Apr 27 2010