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    myelin-oligodendrocyte-glycoprotein-antibody-ep4281-bsa-and-azide-free-ab233025.pdf

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Neuroscience Cell Type Marker Glia marker Oligodendrocyte marker
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RecombinantRabMAb

Recombinant Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
  • Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP4281] to Myelin oligodendrocyte glycoprotein - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Protein
Recombinant Human Myelin oligodendrocyte glycoprotein (ab152080)
Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free
    See all Myelin oligodendrocyte glycoprotein primary antibodies
  • Description

    Rabbit monoclonal [EP4281] to Myelin oligodendrocyte glycoprotein - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Mouse brain, Rat brain and Human brain lysates; Human astrocytoma tissue and Human brain tissue
  • General notes

    Ab233025 is the carrier-free version of ab109746. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab233025 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP4281
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration

Associated products

  • Alternative Versions

    • Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)
    • HRP Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab199236)
    • Alexa Fluor® 647 Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab199472)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Rat brain normal tissue lysate - membrane extract (ab29473)
  • Recombinant Protein

    • Recombinant Human Myelin oligodendrocyte glycoprotein (ab152080)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab233025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.
    • Tissue specificity

      Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. BTN/MOG family.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession Q16653 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4340 Human
      • Entrez Gene: 17441 Mouse
      • Entrez Gene: 24558 Rat
      • Omim: 159465 Human
      • SwissProt: Q16653 Human
      • SwissProt: Q61885 Mouse
      • SwissProt: Q63345 Rat
      • Unigene: 141308 Human
      • Unigene: 210857 Mouse
      • Unigene: 9687 Rat
      see all
    • Alternative names

      • BTN6 antibody
      • BTNL11 antibody
      • MGC26137 antibody
      • MOG alpha 5 antibody
      • MOG alpha 6 antibody
      • MOG AluA antibody
      • MOG AluB antibody
      • MOG antibody
      • MOG Ig AluB antibody
      • MOG_HUMAN antibody
      • MOGIG2 antibody
      • Myelin oligodendrocyte glycoprotein antibody
      • Myelin-oligodendrocyte glycoprotein antibody
      • NRCLP7 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with Purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      ab109746, at 1/1000 dilution, staining Myelin oligodendrocyte glycoprotein in paraffin-embedded Human astrocytoma tissue by Immunohistochemistry. This image was produced using unpurified antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      ab109746, at 1/1000 dilution, staining Myelin oligodendrocyte glycoprotein in paraffin-embedded Human brain tissue by Immunohistochemistry. This image was produced using unpurified antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      ab109746 showing negative staining in Breast carcinoma tissue. This image was produced using unpurified antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      ab109746 showing negative staining in Normal breast tissue. This image was produced using unpurified antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      ab109746 showing positive staining in Glioblastoma tissue. This image was produced using unpurified antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      ab109746 showing negative staining in Colonic adenocarcinoma tissue. This image was produced using unpurified antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      ab109746 showing negative staining in Normal tonsil tissue. This image was produced using unpurified antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      ab109746 showing negative staining in Normal colon tissue. This image was produced using unpurified antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

      ab109746 showing negative staining in Benign prostatic hyperplasia tissue. This image was produced using unpurified antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109746).

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)
      Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - BSA and Azide free (ab233025)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab233025? Please let us know so that we can cite the reference in this datasheet.

    ab233025 has not yet been referenced specifically in any publications.

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