Recombinant
RabMAb

Recombinant Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (HRP) (ab199236)

Overview

  • Product name

    Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (HRP)
    See all Myelin oligodendrocyte glycoprotein primary antibodies
  • Description

    Rabbit monoclonal [EP4281] to Myelin oligodendrocyte glycoprotein (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Myelin oligodendrocyte glycoprotein aa 50-150 (extracellular). The exact sequence is proprietary.

  • Positive control

    • WB: Mouse, rat and human brain tissue lysates. IHC-P: normal human cerebral cortex tissue sections
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab199236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.

Target

  • Function

    Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.
  • Tissue specificity

    Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BTN6 antibody
    • BTNL11 antibody
    • MGC26137 antibody
    • MOG alpha 5 antibody
    • MOG alpha 6 antibody
    • MOG AluA antibody
    • MOG AluB antibody
    • MOG antibody
    • MOG Ig AluB antibody
    • MOG_HUMAN antibody
    • MOGIG2 antibody
    • Myelin oligodendrocyte glycoprotein antibody
    • Myelin-oligodendrocyte glycoprotein antibody
    • NRCLP7 antibody
    see all

Images

  • IHC image of Myelin oligodendrocyte glycoprotein staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab199236, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (HRP) (ab199236) at 1/5000 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199236 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab199236 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab199236.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up