Recombinant
RabMAb

Recombinant Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22629-310] to Myelin oligodendrocyte glycoprotein
  • Suitable for: IHC-P, WB, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310]
    See all Myelin oligodendrocyte glycoprotein primary antibodies

  • Description

    Rabbit monoclonal [EPR22629-310] to Myelin oligodendrocyte glycoprotein

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Mouse Myelin oligodendrocyte glycoprotein aa 1-150. The exact sequence is proprietary.
    Database link: Q61885

  • Positive control

    • WB: Human brain tissue lysate; mouse brain tissue lysate; rat brain tissue lysate. IP: Mouse brain tissue lysate. IHC-P: Human cerebellum tissue; mouse cerebellum tissue; rat cerebellum tissue. IHC-Fr: Mouse cerebellum tissue; rat cerebellum tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab233549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-Fr 1/500.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IP 1/30.

Target

  • Function

    Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.

  • Tissue specificity

    Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BTN6 antibody
    • BTNL11 antibody
    • MGC26137 antibody
    • MOG alpha 5 antibody
    • MOG alpha 6 antibody
    • MOG AluA antibody
    • MOG AluB antibody
    • MOG antibody
    • MOG Ig AluB antibody
    • MOG_HUMAN antibody
    • MOGIG2 antibody
    • Myelin oligodendrocyte glycoprotein antibody
    • Myelin-oligodendrocyte glycoprotein antibody
    • NRCLP7 antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
    Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in rat cerebellum (PMID: 26347141) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

     

  • Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
    Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
    All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 26 seconds; Lane 2: 10 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of rat cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    The section was incubated with ab233549 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
    Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Myelin oligodendrocyte glycoprotein was immunoprecipitated from 0.35 mg of mouse brain tissue lysate whole cell lysate with ab233549 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233549 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 μg (Input).
    Lane 2: ab233549 IP in mouse brain tissue lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233549 in mouse brain tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
    Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
    Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) at 1/1000 dilution + Human brain tissue lysate at 20 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

     

  • Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
    Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in mouse cerebellum (PMID: 26347141) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of mouse cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    The section was incubated with ab233549 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of human cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    The section was incubated with ab233549 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab233549? Please let us know so that we can cite the reference in this datasheet.

ab233549 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted Mar 03 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Cerebellum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted Mar 03 2020

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