Recombinant Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
![Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)](https://www.abcam.com/ps/products/233/ab233549/Images/ab233549-5-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-immunohistochemistry-frozen-cerebellum-rat.jpg "Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22629-310] to Myelin oligodendrocyte glycoprotein
- Suitable for: IHC-P, WB, IHC-Fr, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310]
See all Myelin oligodendrocyte glycoprotein primary antibodies -
Description
Rabbit monoclonal [EPR22629-310] to Myelin oligodendrocyte glycoprotein -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Fr, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain tissue lysate; mouse brain tissue lysate; rat brain tissue lysate. IP: Mouse brain tissue lysate. IHC-P: Human cerebellum tissue; mouse cerebellum tissue; rat cerebellum tissue. IHC-Fr: Mouse cerebellum tissue; rat cerebellum tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22629-310 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| WB |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
|
|
| IHC-Fr |
1/500.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
| IP |
1/30.
|
| Notes |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
|
IHC-Fr
1/500. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
IP
1/30. |
Target
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Function
Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication. -
Tissue specificity
Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4340 Human
- Entrez Gene: 17441 Mouse
- Entrez Gene: 24558 Rat
- Omim: 159465 Human
- SwissProt: Q16653 Human
- SwissProt: Q61885 Mouse
- SwissProt: Q63345 Rat
- Unigene: 141308 Human
see all -
Alternative names
- BTN6 antibody
- BTNL11 antibody
- MGC26137 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in rat cerebellum (PMID: 26347141) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)](https://www.abcam.com/ps/products/233/ab233549/Images/ab233549-8-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-western-blot-brain-lysates.jpg)
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 26 seconds; Lane 2: 10 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)](https://www.abcam.com/ps/products/233/ab233549/Images/ab233549-3-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-immunohistochemistry-cerebellum-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of rat cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab233549 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
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![Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)](https://www.abcam.com/ps/products/233/ab233549/Images/ab233549-6-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-immunoprecipitation-brain-mouse.jpg)
Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)Myelin oligodendrocyte glycoprotein was immunoprecipitated from 0.35 mg of mouse brain tissue lysate whole cell lysate with ab233549 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233549 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 μg (Input).
Lane 2: ab233549 IP in mouse brain tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233549 in mouse brain tissue lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)](https://www.abcam.com/ps/products/233/ab233549/Images/ab233549-7-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-western-blot-brain-lysate-human.jpg)
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) at 1/1000 dilution + Human brain tissue lysate at 20 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)](https://www.abcam.com/ps/products/233/ab233549/Images/ab233549-4-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-immunohistochemistry-frozen-cerebellum-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in mouse cerebellum (PMID: 26347141) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)](https://www.abcam.com/ps/products/233/ab233549/Images/ab233549-2-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-immunohistochemistry-cerebellum-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of mouse cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab233549 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)](https://www.abcam.com/ps/products/233/ab233549/Images/ab233549-1-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-immunohistochemistry-cerebellum-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of human cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab233549 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
-
![Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)](https://www.abcam.com/ps/products/233/ab233549/Images/ab233549-9-benefits-of-recombinant-antibodies.png)
Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab233549 has not yet been referenced specifically in any publications.