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    myelin-plp-antibody-ab28486.pdf

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Neuroscience Cell Adhesion Proteins Membrane Proteins
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Anti-Myelin PLP antibody (ab28486)

  • Datasheet
Reviews (12)Q&A (4)References (37)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody (ab28486)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody (ab28486)

Key features and details

  • Rabbit polyclonal to Myelin PLP
  • Suitable for: IHC-P
  • Reacts with: Mouse
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Myelin PLP antibody [EPR23504-106] (ab254363)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Myelin PLP antibody
    See all Myelin PLP primary antibodies
  • Description

    Rabbit polyclonal to Myelin PLP
  • Host species

    Rabbit
  • Specificity

    ab28486 recognises PLP
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    CG KGLSATVTGG QKGRGSRG

    , corresponding to amino acids 109-128 of Mouse Myelin PLP
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Myelin PLP protein (His tag) (ab252401)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab28486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Mouse
All applications
Cow
Dog
Pig
Macaque monkey
Application Abreviews Notes
IHC-P (2)
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
  • Involvement in disease

    Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
    Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy.
  • Sequence similarities

    Belongs to the myelin proteolipid protein family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P60201 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281410 Cow
    • Entrez Gene: 481002 Dog
    • Entrez Gene: 18823 Mouse
    • SwissProt: P04116 Cow
    • SwissProt: P23294 Dog
    • SwissProt: P60202 Mouse
    • Unigene: 49691 Cow
    • Unigene: 1268 Mouse
    • Alternative names

      • HLD1 antibody
      • Lipophilin antibody
      • Major myelin proteolipid protein antibody
      • MMPL antibody
      • Myelin proteolipid protein antibody
      • MYPR_HUMAN antibody
      • PLP 1 antibody
      • PLP antibody
      • PLP/DM20 antibody
      • PLP1 antibody
      • PMD antibody
      • Proteolipid protein 1 antibody
      • SPG2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody (ab28486)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody (ab28486)

      Immunohistochemical staining of  Myelin PLP in mouse brain thick section using ab28486 (1/1000 dilution). Staining (m) is limited to myelin tracts and oligodendrocytes.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody (ab28486)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody (ab28486)This image is courtesy of an anonymous Abreview
      ab28486 at a 1/1000 dilution staining mouse brain (white matter) by IHC-P. The tissue was fixed in 10% formalin saline. Antigen retrieval was via hydrated autoclaving at 121°C for 15 minutes followed by 5 minutes in 98%formic acid. The antibody was incubated with the tissue for 16 hours and then bound antibody was detected using a biotinylated goat anti-rabbit IgG Fc.

      See Abreview

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (37)

    Publishing research using ab28486? Please let us know so that we can cite the reference in this datasheet.

    ab28486 has been referenced in 37 publications.

    • Tognatta R  et al. Astrocytes Are Required for Oligodendrocyte Survival and Maintenance of Myelin Compaction and Integrity. Front Cell Neurosci 14:74 (2020). PubMed: 32300294
    • Jones RE  et al. Skeletal Stem Cell-Schwann Cell Circuitry in Mandibular Repair. Cell Rep 28:2757-2766.e5 (2019). PubMed: 31509739
    • Farhangi S  et al. In vivo conversion of astrocytes to oligodendrocyte lineage cells in adult mice demyelinated brains by Sox2. Mult Scler Relat Disord 28:263-272 (2019). PubMed: 30639828
    • Radulescu CI  et al. Manipulation of microbiota reveals altered callosal myelination and white matter plasticity in a model of Huntington disease. Neurobiol Dis 127:65-75 (2019). PubMed: 30802499
    • Huang HT  et al. Chronic exposure to high fat diet triggers myelin disruption and interleukin-33 upregulation in hypothalamus. BMC Neurosci 20:33 (2019). PubMed: 31291887
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 16 Abreviews or Q&A

    Western blot abreview for Anti-Myelin PLP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Cortex lysate)
    Gel Running Conditions
    Non-reduced Denaturing (4-20% gels, nitrocellulose membrane)
    Loading amount
    15 µg
    Specification
    Cortex lysate
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 26 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Myelin PLP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (spinal cord)
    Permeabilization
    No
    Specification
    spinal cord
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Tamas Bellak

    Verified customer

    Submitted Nov 27 2017

    Western blot abreview for Anti-Myelin PLP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (brain lysate)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    10 µg
    Specification
    brain lysate
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 28 2017

    Western blot abreview for Anti-Myelin PLP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    100 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Rat Tissue lysate - whole (brain tissue)
    Specification
    brain tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 16 2013

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Myelin PLP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Sample
    Rabbit Tissue sections (brain)
    Specification
    brain
    Permeabilization
    Yes - Acetone
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 09 2013

    ELISA abreview for Anti-Myelin PLP antibody

    Excellent
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Human Cell (brain)
    Specification
    brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Type
    Indirect
    Read More

    Dr. Rachel Barker

    Verified customer

    Submitted Nov 06 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myelin PLP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (brain)
    Specification
    brain
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Citrate
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More

    Dr. Rachel Barker

    Verified customer

    Submitted Nov 06 2012

    Question

    No bands in Western blot with rat brain lysates.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 18 2012

    Answer

    Thank you very much for your call today and for letting us know about the trouble with this antibody.

    As we discussed, I'm sending a free of charge vial from a new lot on the order ***, which should arrive tomorrow.

    Please keep me updated about the results with this new lot. We do fully guarantee the results with this replacement lot, and if it also does not work I'd be happy to send a different PLP antibody to try.

    Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    Answered on Sep 18 2012

    Question

    No staining in IHC-P despite troubleshooting.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 16 2012

    Answer

    Thank you very much for your call today and for letting us know about the trouble with this antibody.

    As we discussed, I'm sending a free of charge vial of ab105784 on the order ***, which should arrive tomorrow. Please keep me updated about the performance of this replacement antibody, and if there is anything else that we can do for you please let me know.

    Have a nice day.

    Read More

    Abcam Scientific Support

    Answered on Jul 16 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Myelin PLP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (C57B/J mouse Vagus Nerve)
    Specification
    C57B/J mouse Vagus Nerve
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.05% Triton-X100 in PBS
    Blocking step
    5% BSA + 0.5% goat serum in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Mar 01 2012

    1-10 of 16 Abreviews or Q&A

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