Anti-Myelin PLP antibody (ab28486)
Key features and details
- Rabbit polyclonal to Myelin PLP
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Myelin PLP antibody
See all Myelin PLP primary antibodies -
Description
Rabbit polyclonal to Myelin PLP -
Host species
Rabbit -
Specificity
ab28486 recognises PLP -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Cow, Dog, Pig, Macaque monkey
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Immunogen
Synthetic peptide:
CG KGLSATVTGG QKGRGSRG
, corresponding to amino acids 109-128 of Mouse Myelin PLP -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
Use at an assay dependent concentration.
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| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
Target
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Function
This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin. -
Involvement in disease
Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy. -
Sequence similarities
Belongs to the myelin proteolipid protein family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 281410 Cow
- Entrez Gene: 481002 Dog
- Entrez Gene: 18823 Mouse
- SwissProt: P04116 Cow
- SwissProt: P23294 Dog
- SwissProt: P60202 Mouse
- Unigene: 1268 Mouse
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Alternative names
- HLD1 antibody
- Lipophilin antibody
- Major myelin proteolipid protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody (ab28486)
Immunohistochemical staining of Myelin PLP in mouse brain thick section using ab28486 (1/1000 dilution). Staining (m) is limited to myelin tracts and oligodendrocytes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody (ab28486)This image is courtesy of an anonymous Abreviewab28486 at a 1/1000 dilution staining mouse brain (white matter) by IHC-P. The tissue was fixed in 10% formalin saline. Antigen retrieval was via hydrated autoclaving at 121°C for 15 minutes followed by 5 minutes in 98%formic acid. The antibody was incubated with the tissue for 16 hours and then bound antibody was detected using a biotinylated goat anti-rabbit IgG Fc.
Datasheets and documents
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Datasheet download
References (56)
ab28486 has been referenced in 56 publications.
- Huang HT et al. Hericium erinaceus mycelium and its small bioactive compounds promote oligodendrocyte maturation with an increase in myelin basic protein. Sci Rep 11:6551 (2021). PubMed: 33753806
- Mészáros L et al. Human alpha-synuclein overexpressing MBP29 mice mimic functional and structural hallmarks of the cerebellar subtype of multiple system atrophy. Acta Neuropathol Commun 9:68 (2021). PubMed: 33853667
- Huerga-Gómez A et al. ?9 -Tetrahydrocannabinol promotes oligodendrocyte development and CNS myelination in vivo. Glia 69:532-545 (2021). PubMed: 32956517
- Sarn N et al. Germline nuclear-predominant Pten murine model exhibits impaired social and perseverative behavior, microglial activation, and increased oxytocinergic activity. Mol Autism 12:41 (2021). PubMed: 34088332
- Kim HN et al. A Western diet impairs CNS energy homeostasis and recovery after spinal cord injury: Link to astrocyte metabolism. Neurobiol Dis 141:104934 (2020). PubMed: 32376475
