Anti-Myelin PLP antibody (ab28486)
Key features and details
- Rabbit polyclonal to Myelin PLP
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Myelin PLP antibody
See all Myelin PLP primary antibodies -
Description
Rabbit polyclonal to Myelin PLP -
Host species
Rabbit -
Specificity
ab28486 recognises PLP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Mouse
-
Immunogen
Synthetic peptide:
CG KGLSATVTGG QKGRGSRG
, corresponding to amino acids 109-128 of Mouse Myelin PLP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Mouse
|
| All applications |
Cow
Dog
Pig
Macaque monkey
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
Target
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Function
This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin. -
Involvement in disease
Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy. -
Sequence similarities
Belongs to the myelin proteolipid protein family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 281410 Cow
- Entrez Gene: 481002 Dog
- Entrez Gene: 18823 Mouse
- SwissProt: P04116 Cow
- SwissProt: P23294 Dog
- SwissProt: P60202 Mouse
- Unigene: 49691 Cow
- Unigene: 1268 Mouse
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Alternative names
- HLD1 antibody
- Lipophilin antibody
- Major myelin proteolipid protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody (ab28486)
Immunohistochemical staining of Myelin PLP in mouse brain thick section using ab28486 (1/1000 dilution). Staining (m) is limited to myelin tracts and oligodendrocytes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody (ab28486)This image is courtesy of an anonymous Abreviewab28486 at a 1/1000 dilution staining mouse brain (white matter) by IHC-P. The tissue was fixed in 10% formalin saline. Antigen retrieval was via hydrated autoclaving at 121°C for 15 minutes followed by 5 minutes in 98%formic acid. The antibody was incubated with the tissue for 16 hours and then bound antibody was detected using a biotinylated goat anti-rabbit IgG Fc.
Datasheets and documents
References (37)
ab28486 has been referenced in 37 publications.
- Tognatta R et al. Astrocytes Are Required for Oligodendrocyte Survival and Maintenance of Myelin Compaction and Integrity. Front Cell Neurosci 14:74 (2020). PubMed: 32300294
- Jones RE et al. Skeletal Stem Cell-Schwann Cell Circuitry in Mandibular Repair. Cell Rep 28:2757-2766.e5 (2019). PubMed: 31509739
- Farhangi S et al. In vivo conversion of astrocytes to oligodendrocyte lineage cells in adult mice demyelinated brains by Sox2. Mult Scler Relat Disord 28:263-272 (2019). PubMed: 30639828
- Radulescu CI et al. Manipulation of microbiota reveals altered callosal myelination and white matter plasticity in a model of Huntington disease. Neurobiol Dis 127:65-75 (2019). PubMed: 30802499
- Huang HT et al. Chronic exposure to high fat diet triggers myelin disruption and interleukin-33 upregulation in hypothalamus. BMC Neurosci 20:33 (2019). PubMed: 31291887
Customer reviews and Q&As
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