Anti-Myelin PLP antibody (ab28486)
Rabbit polyclonal Myelin PLP antibody. Validated in WB, ELISA, IHC, ICC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Rabbit, Human. Cited in 25 publication(s). Independently reviewed in 12 review(s).
- Datasheet
- References (26)
- Protocols
Overview
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Product nameAnti-Myelin PLP antibody
See all Myelin PLP primary antibodies -
DescriptionRabbit polyclonal to Myelin PLP
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Host speciesRabbit
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Specificityab28486 recognises PLP
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Tested applicationsSuitable for: ELISA, WB, ICC, IHC-FoFr, ICC/IF, IHC-P, IHC-Fr, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Predicted to work with: Cow, Dog, Pig, Macaque monkey
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Immunogen
Synthetic peptide:
CG KGLSATVTGG QKGRGSRG
, corresponding to amino acids 109-128 of Mouse Myelin PLP -
Positive control
- Mouse brain
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: Whole serum -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab28486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/10000. | |
| WB | 1/1000. Predicted molecular weight: 26,30 kDa. | |
| ICC | 1/1000. | |
| IHC-FoFr | 1/100. | |
| ICC/IF | 1/100. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | 1/500. | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 19659692 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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FunctionThis is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
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Involvement in diseaseDefects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy. -
Sequence similaritiesBelongs to the myelin proteolipid protein family.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 281410 Cow
- Entrez Gene: 481002 Dog
- Entrez Gene: 5354 Human
- Entrez Gene: 18823 Mouse
- Entrez Gene: 24943 Rat
- Omim: 300401 Human
- SwissProt: P04116 Cow
- SwissProt: P23294 Dog
see all -
Alternative names
- HLD1 antibody
- Lipophilin antibody
- Major myelin proteolipid protein antibody
see all
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin PLP antibody (ab28486)This image is courtesy of an Abreview submitted by Nagendra Rai Nagendraab28486 staining Myelin PLP in Rabbit brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with methanol, permeablized with acetone and blocked with 5% BSA for 1 hour at 37°C. The sample was incubated with primary antibody (1/100) at 4°C for 18 hours. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody (ab28486)This image is courtesy of an Abreview submitted by Dr Rachel BarkerImmunofluorescence analysis of Human brain cells, staining Myelin PLP with ab28486.
Cells were fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/400 in diluent) for 16 hours. An AlexaFluor®555-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody (ab28486)Immunohistochemical staining of Myelin PLP in mouse brain thick section using ab28486 (1/1000 dilution). Staining (m) is limited to myelin tracts and oligodendrocytes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody (ab28486)This image is courtesy of an anonymous Abreviewab28486 at a 1/1000 dilution staining mouse brain (white matter) by IHC-P. The tissue was fixed in 10% formalin saline. Antigen retrieval was via hydrated autoclaving at 121°C for 15 minutes followed by 5 minutes in 98%formic acid. The antibody was incubated with the tissue for 16 hours and then bound antibody was detected using a biotinylated goat anti-rabbit IgG Fc.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Farhangi S et al. In vivo conversion of astrocytes to oligodendrocyte lineage cells in adult mice demyelinated brains by Sox2. Mult Scler Relat Disord 28:263-272 (2019). Read more (PubMed: 30639828) »
- Luo F et al. Oligodendrocyte-specific loss of Cdk5 disrupts the architecture of nodes of Ranvier as well as learning and memory. Exp Neurol 306:92-104 (2018). Read more (PubMed: 29729246) »
