Immunohistochemistry (Frozen sections) abreview for Anti-Myelin PLP antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (C57B/J mouse Vagus Nerve)
Specification
C57B/J mouse Vagus Nerve
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton-X100 in PBS
Blocking step
5% BSA + 0.5% goat serum in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBST

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Additional data

Additional Notes
Frozen cryosections were generated for the Vagus nerve of from C57B/J mice. Sections were permeablized, blocked, and incubated overnight with the primary antibody. The primary antibodies were detected with an HRP-conjugated secondary antibody. The antibody produces the expect staining pattern in these sections.

Dr. Kirk Mcmanus

Verified customer

Submitted Mar 01 2012

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