Western blot abreview for Anti-Myelin PLP antibody

Excellent
Abreviews
Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Rat Tissue lysate - whole (brain tissue)
Specification
brain tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
FEMTO
Exposure
30 second(s)
Negative control
BSA
Positive control
BRAIN TISSUE LYSATE

Additional data

Additional Notes
FEMTO A THERMO PRODUCT
PubMed ID
23680456: View

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Submitted Sep 16 2013

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