Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin PLP antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain, White matter)
Specification
Brain, White matter
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5%

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG-Fc affinity purified
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Fixed in 10% formalin saline. Antigen retrieval via hydrated autoclaving at 121 degrees celsius for 15 minutes, followed by 5 minutes in 98% Formic acid. Peroxidase quench with 2% Hydrogen peroxide in Methanol for 10 minutes.

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Submitted Jul 05 2006

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