Western blot abreview for Anti-Myelin PLP antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (brain lysate)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
10 µg
Specification
brain lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti-Rabbit-IgG HRP linked (Cell Signaling)
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL prime
Negative control
liver
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 25 kDa
Positive control
brain

Abcam user community

Verified customer

Submitted Sep 28 2017

Sign up