Western blot abreview for Anti-Myelin PLP antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Cortex lysate)
Gel Running Conditions
Non-reduced Denaturing (4-20% gels, nitrocellulose membrane)
Loading amount
15 µg
Specification
Cortex lysate
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0,2% Tween + 2,5% BSA
Dilution
1/2000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 24, 40 kDa

Additional data

Additional Notes
Good antibody that works well in WB. Blocking with 5% BSA (milk doest not work) Denaturation should not be done at more than 70°C as the second isoform (DM20, 25kD) will not be detected (at high temperatures myelin aggregates)

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Submitted Mar 26 2018

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