• Product name
    Anti-Myelin PLP antibody [plpc 1]
    See all Myelin PLP primary antibodies
  • Description
    Mouse monoclonal [plpc 1] to Myelin PLP
  • Host species
  • Specificity
    This antibody recognizes myelin proteolipid protein
  • Tested applications
    Suitable for: IHC-FoFr, IHC-Fr, WB, ICC/IF, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 272-277 of Myelin PLP.



Our Abpromise guarantee covers the use of ab9311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 17626200
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF 1/100.
IHC (PFA fixed) 1/1000.


  • Function
    This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
  • Involvement in disease
    Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
    Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy.
  • Sequence similarities
    Belongs to the myelin proteolipid protein family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • HLD1 antibody
    • Lipophilin antibody
    • Major myelin proteolipid protein antibody
    • MMPL antibody
    • Myelin proteolipid protein antibody
    • MYPR_HUMAN antibody
    • PLP 1 antibody
    • PLP antibody
    • PLP/DM20 antibody
    • PLP1 antibody
    • PMD antibody
    • Proteolipid protein 1 antibody
    • SPG2 antibody
    see all


  • ab9311 at a dilution of 1/1000, staining neuronal Myelin PLP  (Alexa 488 secondary at 1/2000) in rat brain tissue (30µm thick coronal sections) on free floating IHC (see protocol link for detailed description). Image taken with a 40x objective. No labeling observed following omission of primary antibody.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates

  • ab9311 at 1/100 staining rat spinal cord tissue sections by IHC-Fr. The tissue sections were paraformaldehyde fixed and incubated with the antibody for 24 hours. A Texas Red conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview


This product has been referenced in:
  • Göttle P  et al. Teriflunomide promotes oligodendroglial differentiation and myelination. J Neuroinflammation 15:76 (2018). WB . Read more (PubMed: 29534752) »
  • Pereira GB  et al. Targeted deletion of the antisilencer/enhancer (ASE) element from intron 1 of the myelin proteolipid protein gene (Plp1) in mouse reveals that the element is dispensable for Plp1 expression in brain during development and remyelination. J Neurochem 124:454-65 (2013). WB ; Mouse . Read more (PubMed: 23157328) »

See all 9 Publications for this product

Customer reviews and Q&As

For western blotting we recommend a starting dilution of 1/1000 and that users titrate from there to determine the optimal concentration for their individual experiments.

Below please find information regarding your order. Please note it FedEx has delivered this package.
Please feel free to contact us if you have any further questions.

Items ordered:
1 ab9311 Mous...

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I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:


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Thank you for contacting Abcam regarding ab9311.

I am sorry that the customer is experiencing difficulties with this antibody in WB. I have reviewed the protocol information and images and I agree that this antibody is not working as stated ...

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Western blot
Rabbit Tissue lysate - whole (Brain)
Loading amount
20 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-15)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dr. Govindaiah Vinukonda

Verified customer

Submitted Jun 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Rat Cell (Oligodendroglia)
Yes - 0.01% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 07 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Cell lysate - whole cell (oligodendroglia)
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 30 2007

Thank you for your enquiry regarding ab9311. This antibody labels myelin PLP protein therefore it will indeed label the myelin rather than the cell body. I would recommend looking at the product ab7474 (in particular the IHC image) which stains for...

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