Overview

  • Product name

    Anti-Myelin Protein Zero antibody
    See all Myelin Protein Zero primary antibodies
  • Description

    Chicken polyclonal to Myelin Protein Zero
  • Host species

    Chicken
  • Tested applications

    Suitable for: IHC-FoFr, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Two synthetic peptides conjugated to KLH, corresponding to different regions of the Myelin Protein Zero gene product, shared between the Human (NP_000521) and Mouse (NP_032649) sequences.

  • Positive control

    • Adult sciatic nerve
  • General notes

    Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

Properties

Applications

Our Abpromise guarantee covers the use of ab134439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000 - 1/2000. Use 2% paraformaldehyde-fixed tissues.
WB 1/2000 - 1/5000. Predicted molecular weight: 28 kDa.
ICC/IF 1/1000 - 1/2000. Use 2% paraformaldehyde-fixed cells.

Target

  • Function

    Creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae.
  • Tissue specificity

    Found only in peripheral nervous system Schwann cells.
  • Involvement in disease

    Defects in MPZ are the cause of Charcot-Marie-Tooth disease type 1B (CMT1B) [MIM:118200]. CMT1B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet.
    Defects in MPZ are the cause of Charcot-Marie-Tooth disease type 2I (CMT2I) [MIM:607677]. CMT2I is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2I is characterized by late onset (range 47 to 60 years).
    Defects in MPZ are the cause of Charcot-Marie-Tooth disease type 2J (CMT2J) [MIM:607736]. CMT2J is a form of Charcot-Marie-Tooth disease characterized by the association of axonal peripheral neuropathy with hearing loss and pupillary abnormalities such as Adie pupil. Inheritance is autosomal dominant.
    Defects in MPZ are the cause of Adie pupil (ADIEP) [MIM:103100]. A stationary, benign disorder characterized by tonic, sluggishly reacting pupil and hypoactive or absent tendon reflexes. Adie pupil is a characteristic of Charcot-Marie-Tooth disease type 2J.
    Defects in MPZ may be the cause of Charcot-Marie-Tooth disease dominant intermediate type D (CMTDID) [MIM:607791]. CMTDID is a form of Charcot-Marie-Tooth disease characterized by features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
    Defects in MPZ are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
    Defects in MPZ are a cause of congenital hypomyelination neuropathy (CHN) [MIM:605253]. CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness, and very slow nerve conduction velocities.
    Defects in MPZ are a cause of Roussy-Levy syndrome (ROULS) [MIM:180800]; also known as Roussy-Levy hereditary areflexic dystasia. This autosomal dominant disorder resembles Charcot-Marie-Tooth disease type 1 in that it presents with foot deformity, weakness and atrophy of distal limb muscles, especially the peronei, and absent tendon reflexes. The phenotype differs, however, in that it includes static tremor of the upper limbs and gait ataxia.
  • Sequence similarities

    Belongs to the myelin P0 protein family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    N-glycosylated; contains sulfate-substituted glycan.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Charcot Marie Tooth neuropathy 1B antibody
    • CHM antibody
    • CMT1 antibody
    • CMT1B antibody
    • CMT2I antibody
    • CMT2J antibody
    • CMT4E antibody
    • CMTDI3 antibody
    • CMTDID antibody
    • DSS antibody
    • HMSNIB antibody
    • MPP antibody
    • MPZ antibody
    • Myelin peripheral protein antibody
    • Myelin protein P0 antibody
    • Myelin protein zero antibody
    • MYP0_HUMAN antibody
    • P0 antibody
    see all

Images

  • Immunohistochemical analysis of an adult sciatic nerve labelling Myelin Protein Zero in the myelin and Schwann cell processes surrounding the nodes of Ranvier with ab134439 at 1/1000 dilution (green staining). Antibodies against LAMP (lysozome-associated membrane glycoprotein) (red staining) serves as the counterstain, and DAPI (blue staining) allows visualization of nuclei.
  • Anti-Myelin Protein Zero antibody (ab134439) at 1/2000 dilution + Adult mouse (C57 Black) sciatic nerve

    Predicted band size: 28 kDa

  • Immunohistochemical analysis of an adult sciatic nerve labelling Myelin Protein Zero in myelinating Schwann cells with ab134439 at 1/1000 dilution (brown staining).

References

This product has been referenced in:

  • Belanger K  et al. A multi-layered nerve guidance conduit design adapted to facilitate surgical implantation. Health Sci Rep 1:e86 (2018). Read more (PubMed: 30623049) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

I can confirm that this product will interact with the extracellular domain of Myelin Protein Zero.

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