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    myelin-protein-zero-antibody-ab31851.pdf

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Neuroscience Sensory System Somatosensory system Nociception
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Anti-Myelin Protein Zero antibody (ab31851)

  • Datasheet
  • SDS
Reviews (14)Q&A (3)References (38)

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Western blot - Anti-Myelin Protein Zero antibody (ab31851)

    Key features and details

    • Rabbit polyclonal to Myelin Protein Zero
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Myelin Protein Zero antibody
      See all Myelin Protein Zero primary antibodies
    • Description

      Rabbit polyclonal to Myelin Protein Zero
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
      Unsuitable for: IHC-Fr
    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Rat, Human
    • Immunogen

      Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Rat Myelin Protein Zero.

      Read Abcam's proprietary immunogen policy (Peptide available as ab31869.)
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Sensory System
      • Somatosensory system
      • Nociception
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Axon marker

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Recombinant Human Myelin Protein Zero (ab114281)
    • Recombinant Protein

      • Recombinant Human Myelin Protein Zero (ab114281)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab31851 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (5)
    Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
    Application notes
    Is unsuitable for IHC-Fr.

    Target

    • Function

      Creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae.
    • Tissue specificity

      Found only in peripheral nervous system Schwann cells.
    • Involvement in disease

      Defects in MPZ are the cause of Charcot-Marie-Tooth disease type 1B (CMT1B) [MIM:118200]. CMT1B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet.
      Defects in MPZ are the cause of Charcot-Marie-Tooth disease type 2I (CMT2I) [MIM:607677]. CMT2I is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2I is characterized by late onset (range 47 to 60 years).
      Defects in MPZ are the cause of Charcot-Marie-Tooth disease type 2J (CMT2J) [MIM:607736]. CMT2J is a form of Charcot-Marie-Tooth disease characterized by the association of axonal peripheral neuropathy with hearing loss and pupillary abnormalities such as Adie pupil. Inheritance is autosomal dominant.
      Defects in MPZ are the cause of Adie pupil (ADIEP) [MIM:103100]. A stationary, benign disorder characterized by tonic, sluggishly reacting pupil and hypoactive or absent tendon reflexes. Adie pupil is a characteristic of Charcot-Marie-Tooth disease type 2J.
      Defects in MPZ may be the cause of Charcot-Marie-Tooth disease dominant intermediate type D (CMTDID) [MIM:607791]. CMTDID is a form of Charcot-Marie-Tooth disease characterized by features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
      Defects in MPZ are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
      Defects in MPZ are a cause of congenital hypomyelination neuropathy (CHN) [MIM:605253]. CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness, and very slow nerve conduction velocities.
      Defects in MPZ are a cause of Roussy-Levy syndrome (ROULS) [MIM:180800]; also known as Roussy-Levy hereditary areflexic dystasia. This autosomal dominant disorder resembles Charcot-Marie-Tooth disease type 1 in that it presents with foot deformity, weakness and atrophy of distal limb muscles, especially the peronei, and absent tendon reflexes. The phenotype differs, however, in that it includes static tremor of the upper limbs and gait ataxia.
    • Sequence similarities

      Belongs to the myelin P0 protein family.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      N-glycosylated; contains sulfate-substituted glycan.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P25189 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4359 Human
      • Entrez Gene: 17528 Mouse
      • Entrez Gene: 24564 Rat
      • Omim: 159440 Human
      • SwissProt: P25189 Human
      • SwissProt: P27573 Mouse
      • SwissProt: P06907 Rat
      • Unigene: 591486 Human
      • Unigene: 9986 Mouse
      • Unigene: 8997 Rat
      see all
    • Alternative names

      • Charcot Marie Tooth neuropathy 1B antibody
      • CHM antibody
      • CMT1 antibody
      • CMT1B antibody
      • CMT2I antibody
      • CMT2J antibody
      • CMT4E antibody
      • CMTDI3 antibody
      • CMTDID antibody
      • DSS antibody
      • HMSNIB antibody
      • MPP antibody
      • MPZ antibody
      • Myelin peripheral protein antibody
      • Myelin protein P0 antibody
      • Myelin protein zero antibody
      • MYP0_HUMAN antibody
      • P0 antibody
      see all

    Images

    • Western blot - Anti-Myelin Protein Zero antibody (ab31851)
      Western blot - Anti-Myelin Protein Zero antibody (ab31851)
      Anti-Myelin Protein Zero antibody (ab31851) at 1 µg/ml + Mouse Sciatic Nerve Whole Tissue Lysate at 20 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size: 27 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?



      We also see a similar banding pattern in Rat Sciatic Nerve lysate although the band is more smeared then observed in the Western Blot shown here. We believe the smearing is caused by glycosylation of the target protein.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (38)

    Publishing research using ab31851? Please let us know so that we can cite the reference in this datasheet.

    ab31851 has been referenced in 38 publications.

    • Boutary S  et al. Squalenoyl siRNA PMP22 nanoparticles are effective in treating mouse models of Charcot-Marie-Tooth disease type 1 A. Commun Biol 4:317 (2021). PubMed: 33750896
    • Li R  et al. Acidic fibroblast growth factor attenuates type 2 diabetes-induced demyelination via suppressing oxidative stress damage. Cell Death Dis 12:107 (2021). PubMed: 33479232
    • Jiang Y  et al. Basic fibroblast growth factor accelerates myelin debris clearance through activating autophagy to facilitate early peripheral nerve regeneration. J Cell Mol Med 25:2596-2608 (2021). PubMed: 33512767
    • Chen BJ  et al. The transcriptome characteristics of vestibular organs from delayed endolymphatic hydrops patients (Meniere's disease). Clin Otolaryngol 46:823-833 (2021). PubMed: 33655689
    • Smith KE  et al. Divergent membrane properties of mouse cochlear glial cells around hearing onset. J Neurosci Res 99:679-698 (2021). PubMed: 33099767
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 17 Abreviews or Q&A

    Western blot abreview for Anti-Myelin Protein Zero antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Sciatic Nerve)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Sciatic Nerve
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 05 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Myelin Protein Zero antibody

    Average
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 04 2016

    Western blot abreview for Anti-Myelin Protein Zero antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Sciatic nerve)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    20 µg
    Specification
    Sciatic nerve
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 22 2016

    Western blot abreview for Anti-Myelin Protein Zero antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Sciatic nerve)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    20 µg
    Specification
    Sciatic nerve
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 22 2016

    Western blot abreview for Anti-Myelin Protein Zero antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Sciatic nerve)
    Loading amount
    30 µg
    Specification
    Sciatic nerve
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. G.K Sakellariou

    Verified customer

    Submitted May 16 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin Protein Zero antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Jun 26 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin Protein Zero antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Jun 19 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin Protein Zero antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brainstem)
    Specification
    Brainstem
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Jun 19 2012

    Question

    What are the QC testing criteria for ab31851 to prove the specificity?
    Since ab61220 is not available, what other antibody could be used to specifically recognize human Schwann cells and no other cell in PNS/CNS.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 22 2012

    Answer

    Thank you for your call yesterday and for your patience while I've looked into your enquiry.
    I've heard back from the lab scientists about ab31851, and we batch test this antibody in Western blot only with rat and mouse sciatic nerve lysate. All of the passed Western blots are very clean with no background bands, but we don't use any negative control lysates. The immunocytochemistry images on the website are from user-submitted Abreviews, and one reviewer did use a double conditional knockout (in Schwann cells) which seems to confirm specificity-
    https://www.abcam.com/Myelin-Protein-Zero-antibody-ab31851.html&tab=abreviews&intabreviewid=29426
    Regarding other Schwann cell markers, PMP2 and P0 look like the most specific, and the best alternative marker that I could find in the literature is S100-

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1888031/

    http://deepblue.lib.umich.edu/bitstream/2027.42/47412/1/11068_2005_Article_BF01188409.pdf

    S100 is expressed by glia in the CNS as well, so I'm not sure it will be specific enough for your purposes, but we do have several ICC-validated S100 antibodies such as ab4066-

    https://www.abcam.com/S100-antibody-4C4-9-Astrocyte-Marker-ab4066.html

    Although we don't have any PMP22 antibodies in stock that have been validated in ICC, we do have a couple PMP22 antibodies that may work in this application if you'd be interested in trying one. For example, ab15506 is tested in IHC on frozen sections and FFPE sections, so it will likely work in ICC-

    https://www.abcam.com/PMP22-antibody-ab15506.html

    I am sorry that we don't have exactly what you're looking for at this time, but if you have any questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Aug 22 2012

    Question

    As part of my research, I am using rat Schwann cell (cell line derived from neonatal Wistar rat bought from ECACC).

    I need the primary antibody for myelin sheath/ protein produced by these cells (cultured in vitro).

    I was wondering if you could kindly advice me which one of your product is the most suitable one for my application.

    I look forward to hear from you

    Kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 09 2012

    Answer

    Thank you for contacting us.

    Myelin basic protein and myelin protein Zero are the common membrane associated proteins. Please check the datasheets

    ab7349, ab40390, ab62631, ab53294 etc. and ab31851

    ab128836 is an anti myelin antibody which could be useful for your purpose. Please check the datasheet for a match with your requirements before purchasing these product.

    We do have quite a big range of secondary antibodies and IHC reagents available, please let me know if you would like to have more info.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jul 09 2012

    1-10 of 17 Abreviews or Q&A

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