RecombinantRabMAb

Recombinant Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

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Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
  • Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
  • Immunoprecipitation - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
  • Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
  • Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
  • Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20383] to Myelin Protein Zero
  • Suitable for: IP, WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Myelin Protein Zero antibody [EPR20383]
    See all Myelin Protein Zero primary antibodies

  • Description

    Rabbit monoclonal [EPR20383] to Myelin Protein Zero

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat sciatic nerve lysates. IHC-P: Human peripheral nerves; Mouse and rat sciatic nerves. IHC-Fr: Mouse and rat sciatic nerves.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/20.
WB
1/1000. Detects a band of approximately 26-28 kDa (predicted molecular weight: 28 kDa).
IHC-P (1)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/50.
Notes
IP
1/20.
WB
1/1000. Detects a band of approximately 26-28 kDa (predicted molecular weight: 28 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/50.

Target

  • Function

    Creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae.

  • Tissue specificity

    Found only in peripheral nervous system Schwann cells.

  • Involvement in disease

    Defects in MPZ are the cause of Charcot-Marie-Tooth disease type 1B (CMT1B) [MIM:118200]. CMT1B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet.
    Defects in MPZ are the cause of Charcot-Marie-Tooth disease type 2I (CMT2I) [MIM:607677]. CMT2I is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2I is characterized by late onset (range 47 to 60 years).
    Defects in MPZ are the cause of Charcot-Marie-Tooth disease type 2J (CMT2J) [MIM:607736]. CMT2J is a form of Charcot-Marie-Tooth disease characterized by the association of axonal peripheral neuropathy with hearing loss and pupillary abnormalities such as Adie pupil. Inheritance is autosomal dominant.
    Defects in MPZ are the cause of Adie pupil (ADIEP) [MIM:103100]. A stationary, benign disorder characterized by tonic, sluggishly reacting pupil and hypoactive or absent tendon reflexes. Adie pupil is a characteristic of Charcot-Marie-Tooth disease type 2J.
    Defects in MPZ may be the cause of Charcot-Marie-Tooth disease dominant intermediate type D (CMTDID) [MIM:607791]. CMTDID is a form of Charcot-Marie-Tooth disease characterized by features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
    Defects in MPZ are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
    Defects in MPZ are a cause of congenital hypomyelination neuropathy (CHN) [MIM:605253]. CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness, and very slow nerve conduction velocities.
    Defects in MPZ are a cause of Roussy-Levy syndrome (ROULS) [MIM:180800]; also known as Roussy-Levy hereditary areflexic dystasia. This autosomal dominant disorder resembles Charcot-Marie-Tooth disease type 1 in that it presents with foot deformity, weakness and atrophy of distal limb muscles, especially the peronei, and absent tendon reflexes. The phenotype differs, however, in that it includes static tremor of the upper limbs and gait ataxia.

  • Sequence similarities

    Belongs to the myelin P0 protein family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    N-glycosylated; contains sulfate-substituted glycan.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Charcot Marie Tooth neuropathy 1B antibody
    • CHM antibody
    • CMT1 antibody
    • CMT1B antibody
    • CMT2I antibody
    • CMT2J antibody
    • CMT4E antibody
    • CMTDI3 antibody
    • CMTDID antibody
    • DSS antibody
    • HMSNIB antibody
    • MPP antibody
    • MPZ antibody
    • Myelin peripheral protein antibody
    • Myelin protein P0 antibody
    • Myelin protein zero antibody
    • MYP0_HUMAN antibody
    • P0 antibody
    see all

Images

  • Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
    Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
    All lanes : Anti-Myelin Protein Zero antibody [EPR20383] (ab183868) at 1/5000 dilution

    Lane 1 : Mouse sciatic nerve lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat sciatic nerve lysate
    Lane 5 : Rat liver lysate
    Lane 6 : Rat brain lysate

    Lysates/proteins at 4 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa
    Observed band size: 26-28 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The MW observed is consistent with the literature (PMID 10704451; PMID 1384988).

    Negative control: liver, brain tissue (PMID 2578885, PMID 25288117).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

    Immunohistochemical analysis of paraffin-embedded human peripheral nerves labeling Myelin Protein Zero with ab183868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Membranous staining on Schwann cells of human peripheral nerves (PMID: 21057508).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
    Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Membranous staining on Schwann cells of mouse sciatic nerves (PMID: 21057508).

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunoprecipitation - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
    Immunoprecipitation - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

    Myelin Protein Zero was immunoprecipitated from Human Sciatic Nerve lysate with ab183868 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab183868 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: Human Sciatic Nerve lysate 10ug (Input).

    Lane 2: ab183868 IP in Human Sciatic Nerve lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183868 in Human Sciatic Nerve lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

    Immunohistochemical analysis of paraffin-embedded mouse sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Membranous staining on Schwann cells of mouse sciatic nerves (PMID: 21057508).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
    Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
    All lanes : Anti-Myelin Protein Zero antibody [EPR20383] (ab183868) at 1/1000 dilution

    Lane 1 : Human sciatic nerve lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa
    Observed band size: 26-28 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The MW observed is consistent with the literature (PMID 10704451; PMID 1384988).

    Negative control: liver tissue (PMID 2578885).

  • Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
    Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Membranous staining on Schwann cells of rat sciatic nerves (PMID: 21057508).

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

    Immunohistochemical analysis of paraffin-embedded rat sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Membranous staining on Schwann cells of rat sciatic nerves (PMID: 21057508).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
    Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

References (2)

Publishing research using ab183868? Please let us know so that we can cite the reference in this datasheet.

ab183868 has been referenced in 2 publications.

  • Bonnet M  et al. Immediate or Delayed Transplantation of a Vein Conduit Filled with Nasal Olfactory Stem Cells Improves Locomotion and Axogenesis in Rats after a Peroneal Nerve Loss of Substance. Int J Mol Sci 21:N/A (2020). PubMed: 32290426
  • Elsayed H  et al. Development and Characterisation of an in vitro Model of Wallerian Degeneration. Front Bioeng Biotechnol 8:784 (2020). PubMed: 32754584

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin Protein Zero antibody [EPR20383]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Vagus Nerve)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab93684 Tris-EDTA Buffer pH 9.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Vagus Nerve
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
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Mr. David Ivancic

Verified customer

Submitted Jun 20 2019

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