• Product name
    Anti-Myeloid leukemia factor 1 antibody
    See all Myeloid leukemia factor 1 primary antibodies
  • Description
    Rabbit polyclonal to Myeloid leukemia factor 1
  • Host species
  • Specificity
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A region between residue 200 and the C-terminus (residue 256) of human Myeloid leukemia factor 1.

  • Positive control
    • Whole cell lysate from HeLa cells.



Our Abpromise guarantee covers the use of ab70211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/5000 - 1/15000. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
    • Tissue specificity
      Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.
    • Involvement in disease
      Note=A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM.
    • Sequence similarities
      Belongs to the MLF family.
    • Post-translational
      Phosphorylation is required for binding to YWHAZ.
    • Cellular localization
      Cytoplasm. Nucleus. In non-hematopoietic cells, resides primarily in the cytoplasm with some punctate nuclear localization. Shuttles between the cytoplasm and nucleus. In hematopoietic cells, located preferentially in the nucleus. Found in the nucleolus when fused to NPM.
    • Information by UniProt
    • Database links
    • Alternative names
      • Hls7 antibody
      • MLF1 antibody
      • MLF1_HUMAN antibody
      • Myelodysplasia myeloid leukemia factor 1 antibody
      • Myelodysplasia-myeloid leukemia factor 1 antibody
      • Myeloid leukemia factor 1 antibody
      • myeloid leukemia factor 1 variant 1 antibody
      • myeloid leukemia factor 1 variant 2 antibody
      • myeloid leukemia factor 1 variant 3 antibody
      see all


    • All lanes : Anti-Myeloid leukemia factor 1 antibody (ab70211) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 5 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 50 µg

      Predicted band size: 31 kDa
      Observed band size: 35 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 130 kDa, 250 kDa, 28 kDa. We are unsure as to the identity of these extra bands.

    • Detection of Human Myeloid leukemia factor 1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded). Affinity purified rabbit anti-Myeloid leukemia factor 1 antibody (ab70211) was used at 3 µg/mg lysate for IP (Lane 1) and at 0.1 µg/ml for WB detection. Lane 2 represents IP IgG control.


    This product has been referenced in:
    • McDaniel LD  et al. Common variants upstream of MLF1 at 3q25 and within CPZ at 4p16 associated with neuroblastoma. PLoS Genet 13:e1006787 (2017). Read more (PubMed: 28545128) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab70211.
    Please use the links above to contact us or submit feedback about this product.


    Sign up