Anti-Myeloid leukemia factor 1 antibody - N-terminal (ab175534)

Overview

  • Product name
    Anti-Myeloid leukemia factor 1 antibody - N-terminal
    See all Myeloid leukemia factor 1 primary antibodies
  • Description
    Rabbit polyclonal to Myeloid leukemia factor 1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Myeloid leukemia factor 1 aa 41-71 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P58340

  • Positive control
    • Mouse heart lysate; 293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab175534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 31 kDa.

Target

  • Function
    Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
  • Tissue specificity
    Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.
  • Involvement in disease
    Note=A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM.
  • Sequence similarities
    Belongs to the MLF family.
  • Post-translational
    modifications
    Phosphorylation is required for binding to YWHAZ.
  • Cellular localization
    Cytoplasm. Nucleus. In non-hematopoietic cells, resides primarily in the cytoplasm with some punctate nuclear localization. Shuttles between the cytoplasm and nucleus. In hematopoietic cells, located preferentially in the nucleus. Found in the nucleolus when fused to NPM.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hls7 antibody
    • MLF1 antibody
    • MLF1_HUMAN antibody
    • Myelodysplasia myeloid leukemia factor 1 antibody
    • Myelodysplasia-myeloid leukemia factor 1 antibody
    • Myeloid leukemia factor 1 antibody
    • myeloid leukemia factor 1 variant 1 antibody
    • myeloid leukemia factor 1 variant 2 antibody
    • myeloid leukemia factor 1 variant 3 antibody
    see all

Images

  • Anti-Myeloid leukemia factor 1 antibody - N-terminal (ab175534) at 1/1000 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size: 31 kDa

  • All lanes : Anti-Myeloid leukemia factor 1 antibody - N-terminal (ab175534) at 1/1000 dilution

    Lane 1 : 293 non transfected cell lysate
    Lane 2 : 293 Myeloid leukemia factor 1 transfected cell lysate

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 31 kDa

References

ab175534 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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