Key features and details
- Rat monoclonal [ER-MP58] to Myeloid Precursor Cells
- Suitable for: IHC-P, IP, IHC-Fr, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-Myeloid Precursor Cells antibody [ER-MP58]
DescriptionRat monoclonal [ER-MP58] to Myeloid Precursor Cells
Tested applicationsSuitable for: IHC-P, IP, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Mouse
Balb/c macrophage precursor cell hybrids
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab63860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution (PMID 19720836).
IHC-Fr: 1/25 - 1/100.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceER-MP58 is expressed by all bone marrow derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors. The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid committed cells and other haematopoietic progenitors cells in the BM.
Cellular localizationCell Membrane
ab63860 staining Myeloid Precursor Cells in murine lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Lungs were excised, fixed in 10% neutral-buffered formalin and embedded in paraffin. Immunohistochemical analysis was performed with heat-induced antigen retrieval. The primary antibody used was ab63860. A biotinylated secondary antibody was used with and DAB detection kit to reveal the positively stained cells with nuclei counterstained with hematoxylin. Scale bar, 200 µm.
ab63860 has been referenced in 2 publications.
- Karpurapu M et al. Functional PU.1 in macrophages has a pivotal role in NF-?B activation and neutrophilic lung inflammation during endotoxemia. Blood 118:5255-66 (2011). Flow Cyt ; Mouse . PubMed: 21937699
- Lu X & Kang Y Chemokine (C-C motif) ligand 2 engages CCR2+ stromal cells of monocytic origin to promote breast cancer metastasis to lung and bone. J Biol Chem 284:29087-96 (2009). IHC-P ; Mouse . PubMed: 19720836