Overview

  • Product name

    Anti-Myeloid Precursor Cells antibody [ER-MP58]
  • Description

    Rat monoclonal [ER-MP58] to Myeloid Precursor Cells
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-P, IP, IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Balb/c macrophage precursor cell hybrids

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    ER-MP58
  • Myeloma

    Y3/Ag1.2.3
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IP
IHC-Fr
Flow Cyt
  • Application notes
    Flow Cyt: Use 10µl for 106 cells.
    IHC-P: Use at an assay dependent dilution (PMID 19720836).
    IHC-Fr: 1/25 - 1/100.
    IP: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      ER-MP58 is expressed by all bone marrow derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors. The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid committed cells and other haematopoietic progenitors cells in the BM.
    • Cellular localization

      Cell Membrane

    Images

    • ab63860 staining Myeloid Precursor Cells in murine lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      Lungs were excised, fixed in 10% neutral-buffered formalin and embedded in paraffin. Immunohistochemical analysis was performed with heat-induced antigen retrieval. The primary antibody used was ab63860. A biotinylated secondary antibody was used with and DAB detection kit to reveal the positively stained cells with nuclei counterstained with hematoxylin. Scale bar, 200 µm.

    References

    This product has been referenced in:

    • Karpurapu M  et al. Functional PU.1 in macrophages has a pivotal role in NF-?B activation and neutrophilic lung inflammation during endotoxemia. Blood 118:5255-66 (2011). Flow Cyt ; Mouse . Read more (PubMed: 21937699) »
    • Lu X & Kang Y Chemokine (C-C motif) ligand 2 engages CCR2+ stromal cells of monocytic origin to promote breast cancer metastasis to lung and bone. J Biol Chem 284:29087-96 (2009). IHC-P ; Mouse . Read more (PubMed: 19720836) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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