Overview

  • Product name
    Anti-Myeloperoxidase antibody [2C7]
    See all Myeloperoxidase primary antibodies
  • Description
    Mouse monoclonal [2C7] to Myeloperoxidase
  • Host species
    Mouse
  • Specificity
    ab25989 recognises myeloperoxidase (MPO).
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Myeloperoxidase.

Properties

Applications

Our Abpromise guarantee covers the use of ab25989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20818377
IHC-Fr 1/1000 - 1/5000.
IHC-P 1/500 - 1/1000.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 84 kDa.
Flow Cyt 1/20 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in disease
    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similarities
    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab25989 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25989, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References

This product has been referenced in:
  • Khan MA  et al. Regulating NETosis: Increasing pH Promotes NADPH Oxidase-Dependent NETosis. Front Med (Lausanne) 5:19 (2018). Read more (PubMed: 29487850) »
  • Nadesalingam A  et al. Hypertonic Saline Suppresses NADPH Oxidase-Dependent Neutrophil Extracellular Trap Formation and Promotes Apoptosis. Front Immunol 9:359 (2018). Read more (PubMed: 29593709) »
See all 27 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (LIVER)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ph6 (ER1) 20 MINS
Permeabilization
No
Specification
LIVER
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Christopher Bagnall

Verified customer

Submitted Nov 22 2016

Answer

Thank you for contacting us. Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

For MPO, we have tested a pair of antibodies in sandwich ELISA and guarantee them to work in human samples. The 2 are listed below:
capture: ab10165 (https://www.abcam.com/Myeloperoxidase-antibody-4A4-ab10165.html)
detection: ab24467 (https://www.abcam.com/Myeloperoxidase-antibody-2A11-HRP-ab24467.html)

Since sandwich ELISA's can be hit or miss with antibody pairing since each antibody needs to have a unique antigen that it recognizes, going with the pair above is what I recommend since we can guarantee they will work together while we do not guarantee that ab25989 will work with your other antibody. That way, if ab10165 and ab24467 do not work for you in sELISA and we cannot work with you to get them to work we will refund your purchase or send you replacements free of charge.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Answer

DISCOUNT CODE: xxxxxxx Expiration date: 2012-04-20 As discussed I have generated a discount code for ab25989. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for staining of pig samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount

Read More

Answer

Thank you for contacting us and considering our testing discount with ab25989. As the sequence of human Myeloperoxidase shares 86% homology with the pig protein we would predict that  ab25989 would be able to recognise this protein. I can issue a testing discount against this antibody, or if you would like you could choose from the other antibodies we have available against Myeloperoxidase such as rabbit polyclonals ab45977 and ab9535 which have been shown to work well in paraffin embedded tissue sections. More information on the testing dicount can be found here: www.abcam.com/collaborationdiscount If you would like me to issue a testing discount for any of these antibodies please let me know. Once issues, this discount code can be activated once you have submitted an Abreview. More information on how to do this can be found from the following link: www.abcam.com/abreviews We publish both negative and positive Abreviews so your code will be activated regardless of the results of your tests. Once activated you can use your discount code to purchase any primary antibody of your choice from our catalogue free of charge. If you need any further information please do not hesitate to let me know, otherwise I look forward to hearing which antibody you would like to test.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (ventricular tissue lysates)
Loading amount
50 µg
Specification
ventricular tissue lysates
Gel Running Conditions
Non-reduced Denaturing (4~12% gradient NuPAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 05 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (human aortic tissue extract)
Loading amount
30 µg
Specification
human aortic tissue extract
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Dr. Guo-Ping Shi

Verified customer

Submitted Mar 03 2008

Answer

Ab25989 recognizes an epitope on the heavy chain of myeloperoxidase. The protein is a multimer of 2 heavy chains (55kDa) and two light chains (15kDa), the heavy chains being linked by a disulfide bond. In Western blot under non-reducing conditions, the antibody recognizes native MPO (whole molecule) and will recognize the heavy chain following boiling of the sample. Please contact us again if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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