Anti-Myeloperoxidase antibody [2D4] (ab90810)

Reviews (1)Q&A (1)References (5)

Overview

  • Product name
    Anti-Myeloperoxidase antibody [2D4]
    See all Myeloperoxidase primary antibodies
  • Description
    Mouse monoclonal [2D4] to Myeloperoxidase
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, ICC/IF, ELISAmore details
    Unsuitable for: IHC-P or WB
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Full length native protein (purified) corresponding to Myeloperoxidase. Antigen used is purified natural MPO from mice

Properties

Applications

Our Abpromise guarantee covers the use of ab90810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
Flow Cyt 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/50.
ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or WB.

    • Target

    • Function
      Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
    • Involvement in disease
      Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
    • Sequence similarities
      Belongs to the peroxidase family. XPO subfamily.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
      • Alternative names
        • 84 kDa myeloperoxidase antibody
        • 89 kDa myeloperoxidase antibody
        • EC 1.11.1.7 antibody
        • EC1.11.2.2 antibody
        • fj80f04 antibody
        • MPO antibody
        • mpx antibody
        • myeloid-specific peroxidase antibody
        • Myeloperoxidase antibody
        • Myeloperoxidase heavy chain antibody
        • Myeloperoxidase light chain antibody
        • PERM_HUMAN antibody
        • wu:fj80f04 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [2D4] (ab90810)
        Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [2D4] (ab90810)

        ab90810 at 1/50 dilution staining Myeloperoxidase in rat white blood cells by Immunocytochemistry/ Immunofluorescence. Image shows green staining with ab90810 in neutrophils and monocytes.

      References

      This product has been referenced in:
      • Dang G  et al. Extracellular Sphingomyelinase Rv0888 of Mycobacterium tuberculosis Contributes to Pathological Lung Injury of Mycobacterium smegmatis in Mice via Inducing Formation of Neutrophil Extracellular Traps. Front Immunol 9:677 (2018). Read more (PubMed: 29670633) »
      • Gan T  et al. TLR3 Regulated Poly I:C-Induced Neutrophil Extracellular Traps and Acute Lung Injury Partly Through p38 MAP Kinase. Front Microbiol 9:3174 (2018). Read more (PubMed: 30622526) »
      See all 5 Publications for this product

      Publishing research using ab90810? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Myeloperoxidase antibody [2D4]

       Inconclusive
      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Sheep Cell (Lung)
      Specification
      Lung
      Fixative
      Formaldehyde
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted May 13 2013

      Question

      LOT NUMBER -- NOT SPECIFIED -- ORDER NUMBER

      DESCRIPTION OF THE PROBLEM No staining
      SAMPLE Rat ventriclar tissue. 20um cyrosections.
      PRIMARY ANTIBODY Abcam MPO mouse antibody (ab90810). Tried 1:50 and 1:100 dilution. Left in a humidified chamber overnight at +4C.
      DETECTION METHOD ABC method. Applied vectastain Elite ABC kit for 30 minutes at room temperature(prepared according to manufactures instructions).
      POSITIVE AND NEGATIVE CONTROLS USED Do not have access to a positive control, testing control and heart failure sections (expecting to see MPO in heart failure sections). Negative control was tissue with only secondary antibody.
      ANTIBODY STORAGE CONDITIONS Stored at +4C. FIXATION OF SAMPLE Formalin for 30 minutes at room temperature. PERMEABILIZATION STEP 0.1% Trition-X100 for 30 minutes at room temperature.
      BLOCKING CONDITIONS Normal serum (from Vectastain ABC kit)
      SECONDARY ANTIBODY Biotinylated secondary antibody for 60 minutes at room temperature (1.5 mg, Vectastain ABC kit, prepared according to manufactures instructions).
      HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2
      HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No
      WHAT STEPS HAVE YOU ALTERED? DAB incubation time. Initally incubated DAB for 10 minutes but resulted in too much background. Repeated for only 2 minutes but saw no staining.

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      Answer

      Thank you for submitting your completed questionnaire.

      Myeloperoxidase is known to be expressed in a subset of lymphoid cells. Tissues known to express this protein include spleen, lymph node and heatopoietic cells of bone marrow.Any of these tissuescan be usedas positive control. Myeloperoxidase may not be expressed in myocytes.

      I hope this is helpful. Please contact me again if you have any further questions.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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