Anti-Myeloperoxidase antibody [2D4] (ab90810)

Abreviews (1)Q&A (1)Specific References (4)

Overview

  • Product name
    Anti-Myeloperoxidase antibody [2D4]
    See all Myeloperoxidase primary antibodies
  • Description
    Mouse monoclonal [2D4] to Myeloperoxidase
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, ICC/IF, ELISAmore details
    Unsuitable for: IHC-P or WB
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Full length native protein (purified) corresponding to Myeloperoxidase. Antigen used is purified natural MPO from mice

Properties

Applications

Our Abpromise guarantee covers the use of ab90810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
Flow Cyt 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/50.
ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or WB.

    • Target

    • Function
      Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
    • Involvement in disease
      Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
    • Sequence similarities
      Belongs to the peroxidase family. XPO subfamily.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
      • Alternative names
        • 84 kDa myeloperoxidase antibody
        • 89 kDa myeloperoxidase antibody
        • EC 1.11.1.7 antibody
        • EC1.11.2.2 antibody
        • fj80f04 antibody
        • MPO antibody
        • mpx antibody
        • myeloid-specific peroxidase antibody
        • Myeloperoxidase antibody
        • Myeloperoxidase heavy chain antibody
        • Myeloperoxidase light chain antibody
        • PERM_HUMAN antibody
        • wu:fj80f04 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [2D4] (ab90810)
        Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [2D4] (ab90810)

        ab90810 at 1/50 dilution staining Myeloperoxidase in rat white blood cells by Immunocytochemistry/ Immunofluorescence. Image shows green staining with ab90810 in neutrophils and monocytes.

      References

      This product has been referenced in:
      • Dang G  et al. Extracellular Sphingomyelinase Rv0888 of Mycobacterium tuberculosis Contributes to Pathological Lung Injury of Mycobacterium smegmatis in Mice via Inducing Formation of Neutrophil Extracellular Traps. Front Immunol 9:677 (2018). Read more (PubMed: 29670633) »
      • Abdollahi M  et al. Insulin treatment prevents wounding associated changes in tissue and circulating neutrophil MMP-9 and NGAL in diabetic rats. PLoS One 12:e0170951 (2017). ICC/IF ; Rat . Read more (PubMed: 28182694) »

      See all 4 Publications for this product

      Publishing research using ab90810? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      2 reviews or Q&A

      10
      Votes: Highest First

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Myeloperoxidase antibody [2D4]

       Inconclusive
      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Sheep Cell (Lung)
      Specification
      Lung
      Fixative
      Formaldehyde
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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      Username

      Abcam user community

      Verified customer

      Submitted May 13 2013

      LOT NUMBER -- NOT SPECIFIED -- ORDER NUMBER

      DESCRIPTION OF THE PROBLEM No staining
      SAMPLE Rat ventriclar tissue. 20um cyrosections.
      PRIMARY ANTIBODY Abcam MPO mouse antibody (ab90810). Tried 1:50 and 1:100 dilution. Left in a humid...

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      Thank you for submitting your completed questionnaire.

      Myeloperoxidase is known to be expressed in a subset of lymphoid cells. Tissues known to express this protein include spleen, lymph node and heatopoietic cells of bone marrow.Any of these...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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