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  1. Link

    myeloperoxidase-antibody-2d4-ab90810.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Myeloperoxidase antibody [2D4] (ab90810)

  • Datasheet
Reviews (1)Q&A (1)References (7)

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  • Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [2D4] (ab90810)

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Primary

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  • Datasheet
  • References (7)
  • Protocols

Overview

  • Product name

    Anti-Myeloperoxidase antibody [2D4]
    See all Myeloperoxidase primary antibodies
  • Description

    Mouse monoclonal [2D4] to Myeloperoxidase
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, ICC/IF, ELISAmore details
    Unsuitable for: IHC-P or WB
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Full length native protein (purified) corresponding to Myeloperoxidase. Antigen used is purified natural MPO from mice

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab90810 is provided as a 0.2 µm filtered solution.
  • Clonality

    Monoclonal
  • Clone number

    2D4
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Other
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
    • Arterial
    • Cardiovascular
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    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-Myeloperoxidase antibody [2D4] (Biotin) (ab90811)
    • Anti-Myeloperoxidase antibody [2D4] (FITC) (ab90812)
  • Assay kits

    • Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (ab105136)
    • Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Myeloperoxidase protein (ab158915)

Applications

Our Abpromise guarantee covers the use of ab90810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
Flow Cyt 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/50.
ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or WB.
  • Target

    • Function

      Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
    • Involvement in disease

      Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
    • Sequence similarities

      Belongs to the peroxidase family. XPO subfamily.
    • Cellular localization

      Lysosome.
    • Target information above from: UniProt accession P05164 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 17523 Mouse
      • Entrez Gene: 303413 Rat
      • SwissProt: P11247 Mouse
      • Unigene: 4668 Mouse
      • Unigene: 47782 Rat
      • Alternative names

        • 84 kDa myeloperoxidase antibody
        • 89 kDa myeloperoxidase antibody
        • EC 1.11.1.7 antibody
        • EC1.11.2.2 antibody
        • fj80f04 antibody
        • MPO antibody
        • mpx antibody
        • myeloid-specific peroxidase antibody
        • Myeloperoxidase antibody
        • Myeloperoxidase heavy chain antibody
        • Myeloperoxidase light chain antibody
        • PERM_HUMAN antibody
        • wu:fj80f04 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [2D4] (ab90810)
        Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [2D4] (ab90810)

        ab90810 at 1/50 dilution staining Myeloperoxidase in rat white blood cells by Immunocytochemistry/ Immunofluorescence. Image shows green staining with ab90810 in neutrophils and monocytes.

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Datasheets and documents

      • Datasheet
    • References

      This product has been referenced in:

      • Wang N  et al. Neutrophil extracellular traps induced by VP1 contribute to pulmonary edema during EV71 infection. Cell Death Discov 5:111 (2019). Read more (PubMed: 31285854) »
      • Dang G  et al. Extracellular Sphingomyelinase Rv0888 of Mycobacterium tuberculosis Contributes to Pathological Lung Injury of Mycobacterium smegmatis in Mice via Inducing Formation of Neutrophil Extracellular Traps. Front Immunol 9:677 (2018). Read more (PubMed: 29670633) »
      See all 7 Publications for this product

      Publishing research using ab90810? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Myeloperoxidase antibody [2D4]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Sheep Cell (Lung)
      Specification
      Lung
      Fixative
      Formaldehyde
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted May 13 2013

      Question

      LOT NUMBER -- NOT SPECIFIED -- ORDER NUMBER

      DESCRIPTION OF THE PROBLEM No staining
      SAMPLE Rat ventriclar tissue. 20um cyrosections.
      PRIMARY ANTIBODY Abcam MPO mouse antibody (ab90810). Tried 1:50 and 1:100 dilution. Left in a humidified chamber overnight at +4C.
      DETECTION METHOD ABC method. Applied vectastain Elite ABC kit for 30 minutes at room temperature(prepared according to manufactures instructions).
      POSITIVE AND NEGATIVE CONTROLS USED Do not have access to a positive control, testing control and heart failure sections (expecting to see MPO in heart failure sections). Negative control was tissue with only secondary antibody.
      ANTIBODY STORAGE CONDITIONS Stored at +4C. FIXATION OF SAMPLE Formalin for 30 minutes at room temperature. PERMEABILIZATION STEP 0.1% Trition-X100 for 30 minutes at room temperature.
      BLOCKING CONDITIONS Normal serum (from Vectastain ABC kit)
      SECONDARY ANTIBODY Biotinylated secondary antibody for 60 minutes at room temperature (1.5 mg, Vectastain ABC kit, prepared according to manufactures instructions).
      HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2
      HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No
      WHAT STEPS HAVE YOU ALTERED? DAB incubation time. Initally incubated DAB for 10 minutes but resulted in too much background. Repeated for only 2 minutes but saw no staining.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 14 2012

      Answer

      Thank you for submitting your completed questionnaire.

      Myeloperoxidase is known to be expressed in a subset of lymphoid cells. Tissues known to express this protein include spleen, lymph node and heatopoietic cells of bone marrow.Any of these tissuescan be usedas positive control. Myeloperoxidase may not be expressed in myocytes.

      I hope this is helpful. Please contact me again if you have any further questions.

      Read More

      Abcam Scientific Support

      Answered on Mar 14 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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