Overview

  • Product name

    Anti-Myeloperoxidase antibody [2D4] (FITC)
    See all Myeloperoxidase primary antibodies
  • Description

    Mouse monoclonal [2D4] to Myeloperoxidase (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or WB
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Purified Rat Myeloperoxidase

Properties

Applications

Our Abpromise guarantee covers the use of ab90812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
Flow Cyt 1/50.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or WB.
  • Target

    • Function

      Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
    • Involvement in disease

      Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
    • Sequence similarities

      Belongs to the peroxidase family. XPO subfamily.
    • Cellular localization

      Lysosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • 84 kDa myeloperoxidase antibody
      • 89 kDa myeloperoxidase antibody
      • EC 1.11.1.7 antibody
      • EC1.11.2.2 antibody
      • fj80f04 antibody
      • MPO antibody
      • mpx antibody
      • myeloid-specific peroxidase antibody
      • Myeloperoxidase antibody
      • Myeloperoxidase heavy chain antibody
      • Myeloperoxidase light chain antibody
      • PERM_HUMAN antibody
      • wu:fj80f04 antibody
      see all

    Images

    • Detection of MPO in Wehi3BD+ cells. Red, black and blue line represent the isotype control, cells only and ab90812 at 20 μg/ml, respectively.

    • Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue labeling Myeloperoxidase with ab90812 (green). 8 µm sections were fixed in 4% paraformaldehyde for 10 min, washed in PBS twice, and then blocked with 20% donkey serum in PBS. β-gal (red), and DAPI (blue). 

    References

    This product has been referenced in:

    • Al-Haidari AA  et al. Neutrophil extracellular traps promote peritoneal metastasis of colon cancer cells. Oncotarget 10:1238-1249 (2019). Read more (PubMed: 30815227) »
    • Gavillet M  et al. A key role for Rac and Pak signaling in neutrophil extracellular traps (NETs) formation defines a new potential therapeutic target. Am J Hematol 93:269-276 (2018). Read more (PubMed: 29124783) »
    See all 11 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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