Overview

  • Product name

    Anti-Myeloperoxidase antibody [8F4]
    See all Myeloperoxidase primary antibodies
  • Description

    Mouse monoclonal [8F4] to Myeloperoxidase
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Purified mouse Myeloperoxidase from WEHI-3 cells.

  • Positive control

    • Neutrophils isolated from digested infarcts

Properties

Applications

Our Abpromise guarantee covers the use of ab16886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/10.
Flow Cyt 1/10.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in disease

    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization

    Lysosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Images

  • Detection of MPO in Wehi3BD+ cells. Red, black and blue line represent the isotype control, cells only and ab16886 at 10 μg/ml, respectively.

References

This product has been referenced in:

  • Roth Flach RJ  et al. Myeloperoxidase inhibition in mice alters atherosclerotic lesion composition. PLoS One 14:e0214150 (2019). Read more (PubMed: 30889221) »
  • Lu SM  et al. HIV-1 Tat-Induced Microgliosis and Synaptic Damage via Interactions between Peripheral and Central Myeloid Cells. PLoS One 6:e23915 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21912650) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat Neutrophils and Human MPO)
Loading amount
6 µg
Specification
Rat Neutrophils and Human MPO
Gel Running Conditions
Reduced Denaturing (7.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5.0% · Temperature: 23°C

Mrs. Donna Flanagan

Verified customer

Submitted Mar 13 2009

Question
Answer

As far as we know, the epitope has not been mapped. Please contact us again if you have any additional questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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