Overview

  • Product name

    Anti-Myeloperoxidase antibody
    See all Myeloperoxidase primary antibodies
  • Description

    Rabbit polyclonal to Myeloperoxidase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Myeloperoxidase aa 650 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: P11247

  • Positive control

    • This antibody gave a positive signal in Mouse Bone Marrow and Mouse Spleen tissue lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal spleen.

Properties

Applications

Our Abpromise guarantee covers the use of ab139748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 81 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in disease

    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization

    Lysosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Images

  • IHC image of Myeloperoxidase staining in Mouse normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab139748, 1µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Myeloperoxidase antibody (ab139748) at 1 µg/ml

    Lane 1 : Mouse Bone Marrow Tissue Lysate
    Lane 2 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 81 kDa
    Observed band size: 63 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 48 kDa (possible cleavage fragment)


    Exposure time: 30 seconds


    The band observed at 64 kDa could potentially be a cleaved form of Myeloperoxidase due to the presence of both a 15 amino acid signal peptide and a 123 amino acid propeptide.

    The band observed at 48 kDa could also represent the Myeloperoxidase heavy chain.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139748 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Dang G  et al. Extracellular Sphingomyelinase Rv0888 of Mycobacterium tuberculosis Contributes to Pathological Lung Injury of Mycobacterium smegmatis in Mice via Inducing Formation of Neutrophil Extracellular Traps. Front Immunol 9:677 (2018). Read more (PubMed: 29670633) »
  • Neumann T  et al. Canonical NF-?B signaling in myeloid cells promotes lung metastasis in a mouse breast cancer model. Oncotarget 9:16775-16791 (2018). Read more (PubMed: 29682184) »
See all 3 Publications for this product

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