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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Myeloperoxidase antibody (ab139748)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (ab139748)
  • Western blot - Anti-Myeloperoxidase antibody (ab139748)

Key features and details

  • Rabbit polyclonal to Myeloperoxidase
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Myeloperoxidase protein (ab158915)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Myeloperoxidase antibody
    See all Myeloperoxidase primary antibodies
  • Description

    Rabbit polyclonal to Myeloperoxidase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Myeloperoxidase aa 650 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: P11247

  • Positive control

    • This antibody gave a positive signal in Mouse Bone Marrow and Mouse Spleen tissue lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal spleen.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Other
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
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Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Myeloperoxidase protein (ab158915)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 81 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 81 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in disease

    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization

    Lysosome.
  • Target information above from: UniProt accession P05164 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 17523 Mouse
    • Entrez Gene: 303413 Rat
    • SwissProt: P11247 Mouse
    • Unigene: 4668 Mouse
    • Alternative names

      • 84 kDa myeloperoxidase antibody
      • 89 kDa myeloperoxidase antibody
      • EC 1.11.1.7 antibody
      • EC1.11.2.2 antibody
      • fj80f04 antibody
      • MPO antibody
      • mpx antibody
      • myeloid-specific peroxidase antibody
      • Myeloperoxidase antibody
      • Myeloperoxidase heavy chain antibody
      • Myeloperoxidase light chain antibody
      • PERM_HUMAN antibody
      • wu:fj80f04 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (ab139748)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (ab139748)

      IHC image of Myeloperoxidase staining in Mouse normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab139748, 1µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-Myeloperoxidase antibody (ab139748)
      Western blot - Anti-Myeloperoxidase antibody (ab139748)
      All lanes : Anti-Myeloperoxidase antibody (ab139748) at 1 µg/ml

      Lane 1 : Mouse Bone Marrow Tissue Lysate
      Lane 2 : Spleen (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 81 kDa
      Observed band size: 63 kDa why is the actual band size different from the predicted?
      Additional bands at: 48 kDa (possible cleavage fragment)


      Exposure time: 30 seconds


      The band observed at 64 kDa could potentially be a cleaved form of Myeloperoxidase due to the presence of both a 15 amino acid signal peptide and a 123 amino acid propeptide.

      The band observed at 48 kDa could also represent the Myeloperoxidase heavy chain.

      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139748 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab139748? Please let us know so that we can cite the reference in this datasheet.

    ab139748 has been referenced in 5 publications.

    • Lei B  et al. Tissue Tropism in Streptococcal Infection: Wild-Type M1T1 Group A Streptococcus Is Efficiently Cleared by Neutrophils Using an NADPH Oxidase-Dependent Mechanism in the Lung but Not in the Skin. Infect Immun 87:N/A (2019). PubMed: 31331954
    • Convente MR  et al. Depletion of Mast Cells and Macrophages Impairs Heterotopic Ossification in an Acvr1R206H Mouse Model of Fibrodysplasia Ossificans Progressiva. J Bone Miner Res 33:269-282 (2018). PubMed: 28986986
    • Dang G  et al. Extracellular Sphingomyelinase Rv0888 of Mycobacterium tuberculosis Contributes to Pathological Lung Injury of Mycobacterium smegmatis in Mice via Inducing Formation of Neutrophil Extracellular Traps. Front Immunol 9:677 (2018). PubMed: 29670633
    • Neumann T  et al. Canonical NF-?B signaling in myeloid cells promotes lung metastasis in a mouse breast cancer model. Oncotarget 9:16775-16791 (2018). PubMed: 29682184
    • Qiao J  et al. Busulfan and cyclosphamide induce liver inflammation through NLRP3 activation in mice after hematopoietic stem cell transplantation. Sci Rep 5:17828 (2015). Mouse . PubMed: 26635145

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