Overview

  • Product name
    Anti-Myeloperoxidase antibody
    See all Myeloperoxidase primary antibodies
  • Description
    Rabbit polyclonal to Myeloperoxidase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig, Monkey
  • Immunogen

    Myeloperoxidase isolated from human granulocytes.

  • Positive control
    • Tonsil IHC-P: Human B cell lymphoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab9535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50.
IHC-Fr 1/100.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in disease
    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similarities
    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Images

  • ab9535 staining Myeloperoxidase in paraffin-embedded rat tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded Immunohistochemistry). Tissue was fixed with formaldehyde and blocked with 20% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody (1/50 in milken) for 1 hour ate 24°C. A Biotin-conjugated goat anti rabbt polyclonal (1/500) was used as secondary antibody.

    See Abreview

  • Ab9535 staining of multinucleated cell (Blue) in human blood smear. Antibody was used at 1/200 for 1 hour at 21°C.

    See Abreview

  • Immunohistochemistry (Frozen sections) of Mouse stomach tissue labelling Myeloperoxidase with ab9535 at 5%.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human B cell lymphoma tissue, staining Myeloperoxidase with ab9535.

References

This product has been referenced in:
  • Chiarot E  et al. Protective effect of Group B Streptococcus type-III polysaccharide conjugates against maternal colonization, ascending infection and neonatal transmission in rodent models. Sci Rep 8:2593 (2018). IHC-P . Read more (PubMed: 29416049) »
  • Jiang MP  et al. ß-arrestin 2 attenuates lipopolysaccharide-induced liver injuryviainhibition of TLR4/NF-?B signaling pathway-mediated inflammation in mice. World J Gastroenterol 24:216-225 (2018). Read more (PubMed: 29375207) »

See all 71 Publications for this product

Customer reviews and Q&As

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Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Hepatocellular carcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0) for 10 min at 95°C
Permeabilization
No
Specification
Hepatocellular carcinoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 22 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
Permeabilization
No
Specification
Colon
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 15 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
Permeabilization
No
Specification
Testis
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 08 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1(Ventana EDTA)
Permeabilization
No
Specification
Colon
Fixative
10% neutral formalin
Username

Abcam user community

Verified customer

Submitted Oct 12 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Intestinal tissue)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Intestinal tissue
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 11 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Mesenteric Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC2/ Citrate buffer ph6
Permeabilization
No
Specification
Mesenteric Lymph node
Fixative
Paraformaldehyde
Username

Dr. Li Li

Verified customer

Submitted May 18 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Username

Mr. Simon Cleary

Verified customer

Submitted May 09 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Normal Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
Permeabilization
No
Specification
Normal Spleen
Blocking step
serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Username

Premier Laboratory

Verified customer

Submitted Apr 11 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (stomach)
Permeabilization
No
Specification
stomach
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Mar 10 2016

1-10 of 33 Abreviews or Q&A

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