Overview

  • Product name
    Anti-Myeloperoxidase antibody
    See all Myeloperoxidase primary antibodies
  • Description
    Rabbit polyclonal to Myeloperoxidase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig, Monkey
  • Immunogen

    Myeloperoxidase isolated from human granulocytes.

  • Positive control
    • IHC-P: Human B cell lymphoma tissue. IHC-Fr: Mouse stomach tissue. ICC/IF: Human blood spear.

Properties

Applications

Our Abpromise guarantee covers the use of ab9535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50.
IHC-Fr 1/100.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in disease
    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similarities
    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Images

  • Evaluation of neutrophilic lung infiltration

    Illustrative microscopic views (magnitude 200x) of immunohistochemistry myeloperoxidase marked lung sections at baseline and at 5 hours after Cushing reflex in the BD and the BD + Corticosteroids pretreated pigs. 

    ab9535 used at 1/100 dilution.

    (After Figure 4 B of Belhaj et al)

  • Ab9535 staining of multinucleated cell (Blue) in human blood smear. Antibody was used at 1/200 for 1 hour at 21°C.

    See Abreview

  • Immunohistochemistry (Frozen sections) of Mouse stomach tissue labelling Myeloperoxidase with ab9535 at 5%.

    See Abreview

  • ab9535 staining Myeloperoxidase in paraffin-embedded rat tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded Immunohistochemistry). Tissue was fixed with formaldehyde and blocked with 20% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody (1/50 in milken) for 1 hour ate 24°C. A Biotin-conjugated goat anti rabbt polyclonal (1/500) was used as secondary antibody.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human B cell lymphoma tissue, staining Myeloperoxidase with ab9535.

References

This product has been referenced in:
  • Chiarot E  et al. Protective effect of Group B Streptococcus type-III polysaccharide conjugates against maternal colonization, ascending infection and neonatal transmission in rodent models. Sci Rep 8:2593 (2018). IHC-P . Read more (PubMed: 29416049) »
  • Jiang MP  et al. ß-arrestin 2 attenuates lipopolysaccharide-induced liver injuryviainhibition of TLR4/NF-?B signaling pathway-mediated inflammation in mice. World J Gastroenterol 24:216-225 (2018). Read more (PubMed: 29375207) »
See all 76 Publications for this product

Customer reviews and Q&As

1-10 of 33 Abreviews or Q&A

Answer

DISCOUNT CODE: XXXXXX
Expiration date: AAAAAAA

I am very pleased to hear your customer would like to test ab9535 Anti-Myeloperoxidase antibody in zebrafish. This code will give them 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for zebrafish and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Hepatocellular carcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0) for 10 min at 95°C
Permeabilization
No
Specification
Hepatocellular carcinoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 22 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
Permeabilization
No
Specification
Colon
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 15 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
Permeabilization
No
Specification
Testis
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 08 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1(Ventana EDTA)
Permeabilization
No
Specification
Colon
Fixative
10% neutral formalin

Abcam user community

Verified customer

Submitted Oct 12 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Intestinal tissue)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Intestinal tissue
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 11 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Mesenteric Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC2/ Citrate buffer ph6
Permeabilization
No
Specification
Mesenteric Lymph node
Fixative
Paraformaldehyde

Dr. Li Li

Verified customer

Submitted May 18 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Formaldehyde

Mr. Simon Cleary

Verified customer

Submitted May 09 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Normal Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
Permeabilization
No
Specification
Normal Spleen
Blocking step
serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF

Premier Laboratory

Verified customer

Submitted Apr 11 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (stomach)
Permeabilization
No
Specification
stomach
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Mar 10 2016

1-10 of 33 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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