Recombinant
RabMAb

Recombinant Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022)

Overview

  • Product name

    Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free
    See all Myeloperoxidase primary antibodies
  • Description

    Rabbit monoclonal [EPR17996] to Myeloperoxidase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse Myeloperoxidase aa 100-300. The exact sequence is proprietary.
    Database link: P11247

  • Positive control

    • IHC-P: Rat spleen tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab236022 is a PBS-only buffer format of ab188211. Please refer to ab188211 for recommended dilutions, protocols, and image data. 

     

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 89, 74, 13 kDa (predicted molecular weight: 83 kDa).

Target

  • Function

    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in disease

    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization

    Lysosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab188211 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neutrophils of human spleen [PMID: 19566938].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188211).

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab188211 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neutrophils of mouse spleen [PMID: 19566938].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188211).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed mouse PBMC labeling Myeloperoxidase with ab188211 at 1/500 dilution (Right) compared with a rabbit monoclonal IgG isotype control (ab172730; Left). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    Mouse peripheral blood mononuclear cells stained intracellularly with ab188211 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188211).

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab188211 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neutrophils of rat spleen [PMID: 19566938].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188211).

References

ab236022 has not yet been referenced specifically in any publications.

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