Overview

  • Product name

    Anti-Myeloperoxidase antibody [EPR4793]
    See all Myeloperoxidase primary antibodies
  • Description

    Rabbit monoclonal [EPR4793] to Myeloperoxidase
  • Host species

    Rabbit
  • Specificity

    The antibody recognizes Myeloperoxidase (85-90 kDa) and its heavy chain (60 kDa).
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of Human Myeloperoxidase

  • Positive control

    • HL 60 cell lysate; Paraffin embedded Human colon, lung and spleen tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
    • Involvement in disease

      Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
    • Sequence similarities

      Belongs to the peroxidase family. XPO subfamily.
    • Cellular localization

      Lysosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • 84 kDa myeloperoxidase antibody
      • 89 kDa myeloperoxidase antibody
      • EC 1.11.1.7 antibody
      • EC1.11.2.2 antibody
      • fj80f04 antibody
      • MPO antibody
      • mpx antibody
      • myeloid-specific peroxidase antibody
      • Myeloperoxidase antibody
      • Myeloperoxidase heavy chain antibody
      • Myeloperoxidase light chain antibody
      • PERM_HUMAN antibody
      • wu:fj80f04 antibody
      see all

    Images

    • Anti-Myeloperoxidase antibody [EPR4793] (ab134132) at 1/1000 dilution + HL 60 cell lysate at 10 µg

      Predicted band size: 83 kDa

    • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Myeloperoxidase with ab134132 at 1/250 dilution
    • Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Myeloperoxidase with ab134132 at 1/250 dilution
    • Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Myeloperoxidase with ab134132 at 1/250 dilution
    • Immunohistochemical analysis of paraffin embedded normal Human lung tissue using ab134132 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab134132 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Bone marrow tissue using ab134132 showing +ve staining.

    References

    This product has been referenced in:

    • Wan J  et al. Inhibition of miR-155 reduces impaired autophagy and improves prognosis in an experimental pancreatitis mouse model. Cell Death Dis 10:303 (2019). Read more (PubMed: 30944299) »
    • Her Z  et al. An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia. J Hematol Oncol 10:162 (2017). IHC-P ; Mouse . Read more (PubMed: 28985760) »
    See all 5 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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