Recombinant
RabMAb

Recombinant Anti-Myeloperoxidase antibody [SP72] (ab93665)

Overview

  • Product name

    Anti-Myeloperoxidase antibody [SP72]
    See all Myeloperoxidase primary antibodies
  • Description

    Rabbit monoclonal [SP72] to Myeloperoxidase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Myeloperoxidase aa 600-700 (C terminal). The exact sequence is proprietary.
    Database link: P05164

  • Epitope

    C terminus
  • Positive control

    • IHC: human tonsil and human spleen tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab93665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. 30 minutes at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
ICC/IF 1/100.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in disease

    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization

    Lysosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling Myeloperoxidase with ab93665 at 1/00 dilution (2.49 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab93665 for 30 mins at room temperature.

  • Overlay histogram showing HeLa cells stained with ab93665 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93665, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil
    tissue sections labeling Myeloperoxidase with ab93665 at 1/00 dilution (2.49 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically positive staining on human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab93665 for 30 mins at room temperature.

  • ab93665, at a 1/100 dilution, staining Myeloperoxidase in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry.
  • ICC/IF image of ab93665 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93665 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab93665 has not yet been referenced specifically in any publications.

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