Product nameMyeloperoxidase (MPO) Activity Assay Kit (Colorimetric)
See all Myeloperoxidase kits
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Homogenate
Assay typeEnzyme activity
Assay time1h 00m
Species reactivityReacts with: Mammals, Other species
Myeloperoxidase (MPO) Activity Assay Kit ab105136 is a rapid, simple, sensitive, and reliable colorimetric assay suitable for use as a high throughput MPO activity assay.
In the MPO assay protocol, myeloperoxidase produces HClO from H2O2 and Cl-. The HClO reacts with taurine to generate the taurine chloramine, which subsequently reacts with the DTNB probe to eliminate color (absorbance at 412 nm). The absorbance is inversely proportional to the amount of MPO enzyme.
This MPO assay kit can be used to detect myeloperoxidase as low as 0.05 mU per well.
MPO assay protocol summary:
- add samples to wells (and assay buffer only to wells for standards)
- add reaction mix to sample wells and incubate for 30 min to 2 hrs
- add stop mix and incubate for 10 min
- add TNB reagent/standard to all wells and incubate for 5-10 min
- analyze with a microplate reader
Myeloperoxidase (MPO) is a peroxidase enzyme (EC 126.96.36.199) most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. Myeloperoxidase contains a heme pigment which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. Myeloperoxidase catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H2O2) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). Myeloperoxidase also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests DTNB Probe Red 1 x 50µl MPO Assay Buffer WM 1 x 25ml MPO Positive Control (lyophilized) Purple 1 vial MPO Substrate Stock Blue 1 x 50µl Stop Mix (lyophilized) Green 1 x 20µl TCEP (50mM) Clear 1 x 50µl
FunctionPart of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Involvement in diseaseDefects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
- Information by UniProt
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 188.8.131.52
This product has been referenced in:
- Namkoong H et al. Clarithromycin expands CD11b+Gr-1+ cells via the STAT3/Bv8 axis to ameliorate lethal endotoxic shock and post-influenza bacterial pneumonia. PLoS Pathog 14:e1006955 (2018). Read more (PubMed: 29621339) »
- Deng W et al. The Circadian Clock Controls Immune Checkpoint Pathway in Sepsis. Cell Rep 24:366-378 (2018). Read more (PubMed: 29996098) »