Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Tissue Extracts
- Sensitivity: 0.0005 mU/well
Product nameMyeloperoxidase (MPO) Activity Assay Kit (Fluorometric)
See all Myeloperoxidase kits
Sample typeCell culture supernatant, Tissue Extracts
Assay typeEnzyme activity
Sensitivity> 0.0005 mU/well
Assay time0h 30m
Species reactivityReacts with: Mammals, Other species
Myeloperoxidase (MPO) Activity Assay Kit ab111749 provides a rapid, simple and reliable fluorometric assay to study MPO activity.
In the MPO assay protocol, MPO catalyzes the production of sodium hypochlorite (NaClO) from hydrogen peroxide (H2O2) and sodium chloride (NaCl). Subsequently, NaClO reacts stoichiometrically with the free radical sensor Aminophenyl fluorescein (APF) to generate fluorescein, whose fluorescence can be detected at Ex/Em = 485/525 nm.
This MPO assay kit can be used to detect MPO activity as low as 0.5 µU per well.
MPO assay protocol summary:
- add fluoroscein standard to wells and analyze with a microplate reader
- add samples to wells
- add reaction mix
- analyze every 2-3 mins with a microplate reader in kinetic mode for at least 30 mins
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Assay Buffer WM 1 x 25ml Fluorescein Standard (1 mM) Yellow cap 1 x 50µl MPO Positive Control Purple cap 1 vial MPO Probe Red cap 1 x 200µl MPO Substrate Stock Blue cap 1 x 50µl
FunctionPart of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Involvement in diseaseDefects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
- Information by UniProt
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 184.108.40.206
ab111749 has been referenced in 6 publications.
- Guenthart BA et al. Regeneration of severely damaged lungs using an interventional cross-circulation platform. Nat Commun 10:1985 (2019). PubMed: 31064987
- Thomas AM et al. Combined Inhibition of C5 and CD14 Attenuates Systemic Inflammation in a Piglet Model of Meconium Aspiration Syndrome. Neonatology 113:322-330 (2018). PubMed: 29486477
- You P et al. Local level of TGF-ß1 determines the effectiveness of dexamethasone through regulating the balance of Treg/Th17 cells in TNBS-induced mouse colitis. Exp Ther Med 15:3639-3649 (2018). PubMed: 29545894
- Kuwabara WMT et al. Obesity and Type 2 Diabetes mellitus induce lipopolysaccharide tolerance in rat neutrophils. Sci Rep 8:17534 (2018). PubMed: 30510205
- Roberts JS et al. Opportunistic Pathogen Porphyromonas gingivalis Modulates Danger Signal ATP-Mediated Antibacterial NOX2 Pathways in Primary Epithelial Cells. Front Cell Infect Microbiol 7:291 (2017). PubMed: 28725637
- Tang Z et al. The role of bradykinin in lung ischemia-reperfusion injury in a rat lung transplantation model. Acta Cir Bras 31:807-812 (2016). PubMed: 28076504