Product nameMyeloperoxidase (MPO) Activity Assay Kit (Fluorometric)
See all Myeloperoxidase kits
Sample typeCell culture supernatant, Tissue Extracts
Assay typeEnzyme activity
Sensitivity> 0.001 mU/well
Assay time0h 30m
Species reactivityReacts with: Mammals, Other species
Myeloperoxidase (MPO) Activity Assay Kit ab111749 provides a rapid, simple and reliable fluorometric assay to study MPO activity.
In the MPO assay protocol, MPO catalyzes the production of sodium hypochlorite (NaClO) from hydrogen peroxide (H2O2) and sodium chloride (NaCl). Subsequently, NaClO reacts stoichiometrically with the free radical sensor Aminophenyl fluorescein (APF) to generate fluorescein, whose fluorescence can be detected at Ex/Em = 485/525 nm.
This MPO assay kit can be used to detect MPO activity as low as 0.5 µU per well.
MPO assay protocol summary:
- add fluoroscein standard to wells and analyze with a microplate reader
- add samples to wells
- add reaction mix
- analyze every 2-3 mins with a microplate reader in kinetic mode for at least 30 mins
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Assay Buffer WM 1 x 25ml Fluorescein Standard (1 mM) Yellow cap 1 x 50µl MPO Positive Control Purple cap 1 vial MPO Probe Red cap 1 x 200µl MPO Substrate Stock Blue cap 1 x 50µl
FunctionPart of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Involvement in diseaseDefects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
- Information by UniProt
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 126.96.36.199
This product has been referenced in:
- Guenthart BA et al. Regeneration of severely damaged lungs using an interventional cross-circulation platform. Nat Commun 10:1985 (2019). Read more (PubMed: 31064987) »
- Thomas AM et al. Combined Inhibition of C5 and CD14 Attenuates Systemic Inflammation in a Piglet Model of Meconium Aspiration Syndrome. Neonatology 113:322-330 (2018). Read more (PubMed: 29486477) »