Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749)

Abreviews (1)Q&A (14)Specific References (2)

Overview

  • Product name
    Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric)
    See all Myeloperoxidase kits
  • Sample type
    Cell culture supernatant, Tissue Extracts
  • Assay type
    Enzyme activity
  • Sensitivity
    > 0.001 mU/well
  • Species reactivity
    Reacts with: Mammals
  • Product overview

    Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749) provides a rapid, simple and reliable method to study MPO activity. MPO catalyzes the production of sodium hypochlorite (NaClO) from hydrogen peroxide (H2O2) and sodium chloride (NaCl). Subsequently, NaClO will react stoichiometrically with the free radical sensor Aminophenyl fluorescein (APF) to generate fluorescein, whose fluorescence can be detected at Ex/Em = 485/525 nm. This kit can be used to detect MPO activity as low as 0.5 µU per well. This kit is suitable for high throughput assay of MPO activity.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as pus and some forms of mucus. Furthermore, it can oxidize tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent.

Properties

Images

  • MPO activity - ab111749
    MPO activity - ab111749

    a. Measurement of MPO activity in WBC lysate (0.1 μg) and MPO Positive Control (3 μL). b. MPO specific activity in WBC lysate.

  • Functional Studies - Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (ab111749)
    Functional Studies - Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (ab111749)
    Standard Curve using this kit protocol (Ex/Em = 485/525 nm)

Protocols

References

This product has been referenced in:
  • You P  et al. Local level of TGF-ß1 determines the effectiveness of dexamethasone through regulating the balance of Treg/Th17 cells in TNBS-induced mouse colitis. Exp Ther Med 15:3639-3649 (2018). Read more (PubMed: 29545894) »
  • Roberts JS  et al. Opportunistic Pathogen Porphyromonas gingivalis Modulates Danger Signal ATP-Mediated Antibacterial NOX2 Pathways in Primary Epithelial Cells. Front Cell Infect Microbiol 7:291 (2017). Read more (PubMed: 28725637) »

See all 2 Publications for this product

Publishing research using ab111749? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Inquiry: Dear Technical Support team: We are interested in measuring MPO activity in mouse liver tissue. I read that Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749) is compatible with tissue extract. Is this kit compatible ...

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Thank you for contacting us.

I would recommend using fresh tissue samples.

Other peroxidases will not be able to produce the same intermediates required for this assay. Therefore it is specific for MPO.


I hope this infor...

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MPO activity of ovine lung tissue

 Good Good 4/5 (Ease of Use)
Abreviews
Application: ELISA - MPO fluorometric assay
Samples: 10mg of homogenized lung tissue in 400uL of assay buffer
Loading amount: NEAT
Followed assay protocol to determine neutrophil infiltration in sheep lung tissue.
Standard curve: very high readings, out of range of neat samples - considered diluting standard to get within reference range
Positive control: was at the bottom end of the standard curve and used as a reference only to check the assay was working correctly
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Ms. Gabriela Simonova

Verified customer

Submitted Sep 03 2014

I have 20 different samples that I want to compare the activity for, so I need to normalize the amount of each sample I'm inputting. Would the assay buffer be compatible with a standard protein concentration measurement (eg. BCA assay)?

The laboratory confirmed that the assay buffer will be compatible with a BCA assay, and this method of normalizing your samples' protein concentration would be appropriate in order to make activity level comparisons between your samples.

We are interested in the fluometric mpo activity assay (ab1117490). We were wondering whether it is it advisable to freeze the tissue from which the mpo activity will be analysed? If yes, do you freeze the tissue directly or do you first homogenize and...

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For peroxidase, fresh samples are the best. If needed the tissue can be quick-frozen in liquid nitrogen and stored at -80C. I would not advise making the lysate and then storing it for best results.

Hi,
I will be using your product ab111749 MPO activity assay kit for mouse organ samples.
Since we have many animals to sacrifice, we wonder if we can keep organ samples in freezer for using this MPO activity kit.
I am considering to nec...

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Thank you for your enquiry regarding ab111749.

We have always used with fresh samples only with this assay. We know of scientists who did store the homogenates in aliquots at -80°C for a short while before use but since we don’t our...

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Customer kindly contacted us regarding MPO activity assay kit (Fluorometric) with a couple questions.
1. Can tissue samples homogenized in assay buffer be frozen at -20 or -80? If so, what buffer conditions are appropriate (protease inhibitors, etc...

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The homogenized samples can be stored in aliquots at -80°C, w/o addition of any other components to the assay buffer. It would be preferable though to use fresh samples.
“V” is the volume of the sample you add to the wells in step ...

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‘Can this MPO assay kit (https://www.abcam.com/Myeloperoxidase-MPO-Activity-Assay-Kit-Fluorometric-ab111749.html) be used for rat tissue homogenates, or is it only for human samples?

This kit can be used with all mammalian samples including rats.

I have updated our datasheet accordingly.

How will EDTA inhibit this assay?

This is an enzymatic activity assay. Enzymes need some ions/cofactors to function and EDTA being a chelator will chelate these co-factors (specifically Mg++ and Ca++), leading to the enzyme becoming non-functional.

What is the concentration of the MPO positive control for my lot?

Thank you for your inquiry and your patience with this reply.

I heard back from the supplying laboratory that the concentration of the positive control MPO does not vary from lot to lot. But unfortunately, they could not disclose the concent...

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Should a black plate with clear bottom wells or opaque bottom wells be used with this kit?


For this assay kit, the client can either use a black opaque bottom plate or, for even more efficiency, use a white opaque bottom plate. One would not use a clear transparent plate.

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