Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate
- Sensitivity: 0.1 mU/ml
Product nameMyeloperoxidase (MPO) Activity Assay Kit (Fluorometric - Red)
See all Myeloperoxidase kits
Sample typeCell Lysate
Assay typeEnzyme activity
Sensitivity< 0.1 mU/ml
Species reactivityReacts with: Mammals, Other species
Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab219925) provides a simple method to measure Myeloperoxidase (MPO) activity in cell lysates. The assay uses our AbRed Indicator to monitor myeloperoxidase (MPO) activity, leading to an increase in fluorescence that can be easily detected at an optimal Ex/Em = 540/590 nm (range: 530-570/590-600 nm). The signal can also be read by absorbance at OD: 576 ± 5 nm, although the sensitivity of the assay is reduced 10-fold.
This assay can detect activity from as low as 0.1 mU/mL Myeloperoxidase (MPO) in solution. The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation or wash step.
Myeloperoxidase (MPO), most abundantly present in neutrophils and monocytes, is a green hemoprotein having peroxidase activity. It catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates; and plays an important role in the oxygen-dependent killing of tumor cells and microorganisms. MPO deficiency is a hereditary deficiency of the enzyme, which predisposes to immune deficiency. However, elevated MPO levels significantly increases the risk for cardiovascular mortality. The fact that circulating levels of MPO have been shown to predict risks for major adverse cardiac events and that levels of MPO derived chlorinated compounds are specific biomarkers for disease progression, has attracted considerable interest in the development of therapeutic MPO inhibitors.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests Assay Buffer 1 x 20ml DMSO 1 x 200µl H2O2 (3%) 1 x 100µl Myeloperoxidase Standard (10 mU) 1 vial Red Dye (light-sensitive) 1 vial
FunctionPart of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Involvement in diseaseDefects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
- Information by UniProt
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 22.214.171.124
ab219925 has not yet been referenced specifically in any publications.