Myeloperoxidase (MPO) Inhibitor Screening Assay Kit (ab133080)

Overview

  • Product name
    Myeloperoxidase (MPO) Inhibitor Screening Assay Kit
    See all Myeloperoxidase kits
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Chlorination 16 3.7%
    Peroxidation 16 2.8%
    Inter-assay
    Sample n Mean SD CV%
    Chlorination 16 3.6%
    Peroxidation 16 3.1%
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammals
  • Product overview

    Abcam’s Myeloperoxidase (MPO) Inhibitor Screening Assay (ab133080) provides convenient fluorescence-based methods for screening inhibitors to both the chlorination and peroxidation activities of MPO. The chlorination assay utilizes the non-fluorescent 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]-benzoic acid (APF), which is selectively cleaved by hypochlorite (-OCl) to yield the highly fluorescent compound fluorescein. Fluorescein fluorescence is analyzed with an excitation wavelength of 480-490 nm and an emission wavelength of 515-520 nm. The peroxidation assay utilizes the peroxidase component of MPO, where a single two electron oxidation of native enzyme (MPO) to compound I (MPO-I) is followed by two successive one electron reductions back to native enzyme by compound II (MPO-II). The reaction between hydrogen peroxide and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence is analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.

Properties

Associated products

Images

  • Inhibition of MPO chlorination activity by 4-aminobenzhydrazide (IC50 = 5 nM).
  • Inhibition of MPO peroxidation activity by 4-aminobenzhydrazide (IC50 = 0.4 µM).

Protocols

References

ab133080 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab133080.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up