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Abcam’s Myeloperoxidase (MPO) Inhibitor Screening Assay (ab133080) provides convenient fluorescence-based methods for screening inhibitors to both the chlorination and peroxidation activities of MPO. The chlorination assay utilizes the non-fluorescent 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]-benzoic acid (APF), which is selectively cleaved by hypochlorite (-OCl) to yield the highly fluorescent compound fluorescein. Fluorescein fluorescence is analyzed with an excitation wavelength of 480-490 nm and an emission wavelength of 515-520 nm. The peroxidation assay utilizes the peroxidase component of MPO, where a single two electron oxidation of native enzyme (MPO) to compound I (MPO-I) is followed by two successive one electron reductions back to native enzyme by compound II (MPO-II). The reaction between hydrogen peroxide and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence is analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
|Components||2 x 96 tests|
|96-Well Plate (black)||2 units|
|96-Well Plate Cover||2 units|
|MPO Assay Buffer||1 vial|
|MPO Chlorination Substrate||1 vial|
|ADHP Assay Reagent||2 vials|
|Myeloperoxidase Assay Reagent||1 vial|
|MPO Inhibitor||1 vial|
|MPO Hydrogen Peroxide||1 vial|
|MPO DMSO||1 x 1ml|
ab133080 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"