Recombinant Anti-Myf5 antibody [EPR4899] (ab125078)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4899] to Myf5
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Myf5 antibody [EPR4899]
See all Myf5 primary antibodies -
Description
Rabbit monoclonal [EPR4899] to Myf5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human skeletal muscle, rat muscle, mouse muscle and rat heart tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4899 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 28 kDa).
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IP |
1/20.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 28 kDa). |
IP
1/20. |
Target
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Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4617 Human
- Entrez Gene: 17877 Mouse
- Entrez Gene: 407603 Pig
- Entrez Gene: 299766 Rat
- Omim: 159990 Human
- SwissProt: P13349 Human
- SwissProt: P24699 Mouse
- Unigene: 178023 Human
see all -
Alternative names
- bHLHc2 antibody
- Class C basic helix loop helix protein 2 antibody
- Class C basic helix-loop-helix protein 2 antibody
see all
Images
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Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution (purified) + Human skeletal muscle tissue lysate at 10 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST -
All lanes : Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution (purified)
Lane 1 : Rat muscle tissue lysate
Lane 2 : Mouse muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST -
ab125078 (Purified) at 1/20 dilution (20 µg/ml) immunoprecipitating Myf5 in Human skeletal muscle lysate.
Lane 1 (input): Human skeletal muscle
Lane 2 (+): 125078 & Human skeletal muscle lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125078 in human skeletal muscle lysate
For western blotting, ab125078 at 1/1000 and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST. -
All lanes : Anti-Myf5 antibody [EPR4899] (ab125078) at 1/10000 dilution (unpurified)
Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Rat muscle tissue lysate
Lane 3 : Mouse muscle tissue lysate
Lane 4 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 28 kDa
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab125078 has been referenced in 9 publications.
- Yang C et al. Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation. Mol Ther Nucleic Acids 23:872-886 (2021). PubMed: 33614236
- Chaudhary P et al. High altitude mediated skeletal muscle atrophy: Protective role of curcumin. Biochimie 156:138-147 (2019). PubMed: 30347230
- Stewart E et al. Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses. Cancer Cell 34:411-426.e19 (2018). PubMed: 30146332
- Skrzypek K et al. SNAIL is a key regulator of alveolar rhabdomyosarcoma tumor growth and differentiation through repression of MYF5 and MYOD function. Cell Death Dis 9:643 (2018). PubMed: 29844345
- Hai T et al. Pilot study of large-scale production of mutant pigs by ENU mutagenesis. Elife 6:N/A (2017). WB ; Pig . PubMed: 28639938
- Llewellyn KJ et al. Myogenic differentiation of VCP disease-induced pluripotent stem cells: A novel platform for drug discovery. PLoS One 12:e0176919 (2017). WB ; Human . PubMed: 28575052
- Tenente IM et al. Myogenic regulatory transcription factors regulate growth in rhabdomyosarcoma. Elife 6:N/A (2017). WB, ICC/IF . PubMed: 28080960
- Koltai E et al. SIRT1 may play a crucial role in overload-induced hypertrophy of skeletal muscle. J Physiol 595:3361-3376 (2017). PubMed: 28251652
- Torrado M et al. Pitx2c is reactivated in the failing myocardium and stimulates myf5 expression in cultured cardiomyocytes. PLoS One 9:e90561 (2014). WB ; Human, Pig . PubMed: 24595098