Anti-Myf5 antibody [EPR4899] (ab125078)
- Datasheet
- Specific References (5)
- Protocols
Overview
-
Product nameAnti-Myf5 antibody [EPR4899]
See all Myf5 primary antibodies -
DescriptionRabbit monoclonal [EPR4899] to Myf5
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Pig
-
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Myf5 aa 200 to the C-terminus. The exact sequence is proprietary.
-
Positive control
- WB: Human skeletal muscle, rat muscle, mouse muscle and rat heart tissue lysate.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
-
ClonalityMonoclonal
-
Clone numberEPR4899
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab125078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 28 kDa). | |
| IP | Use at an assay dependent concentration. |
Target
-
FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
-
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
-
Cellular localizationNucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 4617 Human
- Entrez Gene: 17877 Mouse
- Entrez Gene: 407603 Pig
- Entrez Gene: 299766 Rat
- Omim: 159990 Human
- SwissProt: P13349 Human
- SwissProt: P24699 Mouse
- Unigene: 178023 Human
see all -
Alternative names
- bHLHc2 antibody
- Class C basic helix loop helix protein 2 antibody
- Class C basic helix-loop-helix protein 2 antibody
see all
Images
-
![Western blot - Anti-Myf5 antibody [EPR4899] (ab125078)](https://www.abcam.com/ps/products/125/ab125078/Images/ab125078-4-ab125078WB1.jpg)
Western blot - Anti-Myf5 antibody [EPR4899] (ab125078)Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution (purified) + Human skeletal muscle tissue lysate at 10 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 39 kDa (why is the actual band size different from the predicted?)
Blocking and dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Myf5 antibody [EPR4899] (ab125078)](https://www.abcam.com/ps/products/125/ab125078/Images/ab125078-5-ab125078WB2.jpg)
Western blot - Anti-Myf5 antibody [EPR4899] (ab125078)All lanes : Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution (purified)
Lane 1 : Rat muscle tissue lysate
Lane 2 : Mouse muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 39 kDa (why is the actual band size different from the predicted?)
Blocking and dilution buffer: 5% NFDM/TBST -
![Immunoprecipitation - Anti-Myf5 antibody [EPR4899] (ab125078)](https://www.abcam.com/ps/products/125/ab125078/Images/ab125078-308589-ab125078IP.jpg)
Immunoprecipitation - Anti-Myf5 antibody [EPR4899] (ab125078)Ab125078 (Purified) at 1:1000 dilution (0.666 µg/ml) immunoprecipitating Myf5 in Human skeletal muscle lysate.
Lane 1 (input): Human skeletal muscle
Lane 2 (+): 125078 & Human skeletal muscle lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125078 in human skeletal muscle lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST. -
![Western blot - Anti-Myf5 antibody [EPR4899] (ab125078)](https://www.abcam.com/ps/products/125/ab125078/Images/Myf5-Primary-antibodies-ab125078-1.jpg)
Western blot - Anti-Myf5 antibody [EPR4899] (ab125078)All lanes : Anti-Myf5 antibody [EPR4899] (ab125078) at 1/10000 dilution (unpurified)
Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Rat muscle tissue lysate
Lane 3 : Mouse muscle tissue lysate
Lane 4 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 28 kDa
References
This product has been referenced in:
- Skrzypek K et al. SNAIL is a key regulator of alveolar rhabdomyosarcoma tumor growth and differentiation through repression of MYF5 and MYOD function. Cell Death Dis 9:643 (2018). Read more (PubMed: 29844345) »
- Hai T et al. Pilot study of large-scale production of mutant pigs by ENU mutagenesis. Elife 6:N/A (2017). WB ; Pig . Read more (PubMed: 28639938) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"