Product nameAnti-MYF6 antibody
See all MYF6 primary antibodies
DescriptionRabbit polyclonal to MYF6
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Mouse, Human
3 synthetic peptides (human) conjugated to KLH
- Purchase matching WB positive control:Recombinant Human MYF6 protein
- Induced culture of E.coli (one shot Top10 competent cells).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesCrude serum, final bleed.
Our Abpromise guarantee covers the use of ab82842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/17000 is determined for one of the three peptides.
WB: 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Tissue specificitySkeletal muscle.
Involvement in diseaseDefects in MYF6 may be a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Information by UniProt
- bHLHc4 antibody
- Class C basic helix-loop-helix protein 4 antibody
- CNM3 antibody
All lanes : Anti-MYF6 antibody (ab82842) at 1/1000 dilution
Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
Lane 2 : induced culture of E.coli (one shot Top10 competent cells)
All lanes : anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Additional bands at: 60 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Plasmid name: pBAD-DEST49.
Predicted molecular weight is 27kDa, tag is 14kDa.