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    myh1-antibody-ab127539.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
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Anti-MYH1 antibody (ab127539)

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Western blot - Anti-MYH1 antibody (ab127539)

    Key features and details

    • Rabbit polyclonal to MYH1
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Myosin Heavy Chain/MHC antibody [A4.1025] (ab37484)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-MYH1 antibody
      See all MYH1 primary antibodies
    • Description

      Rabbit polyclonal to MYH1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Rat, Chicken, Cow, Dog, Human, Pig, Monkey
    • Immunogen

      Recombinant fragment, corresponding to a region within N terminal amino acids 51-233 of Human MYH1 (P12882).

    • Positive control

      • Mouse heart whole cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.025% Proclin 300
      Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Motor Proteins
      • Myosin

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Mouse heart normal tissue lysate - total protein (ab30291)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab127539 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 223 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 223 kDa.

    Target

    • Function

      Muscle contraction.
    • Sequence similarities

      Contains 1 IQ domain.
      Contains 1 myosin head-like domain.
    • Domain

      The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
      Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
    • Cellular localization

      Cytoplasm > myofibril. Thick filaments of the myofibrils.
    • Target information above from: UniProt accession P12882 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 417309 Chicken
      • Entrez Gene: 281337 Cow
      • Entrez Gene: 100135061 Dog
      • Entrez Gene: 4619 Human
      • Entrez Gene: 17879 Mouse
      • Entrez Gene: 100125538 Pig
      • Entrez Gene: 287408 Rat
      • Omim: 160730 Human
      • SwissProt: Q9BE40 Cow
      • SwissProt: Q076A6 Dog
      • SwissProt: P12882 Human
      • SwissProt: Q5SX40 Mouse
      • SwissProt: Q9TV61 Pig
      • Unigene: 689619 Human
      • Unigene: 477065 Mouse
      see all
    • Alternative names

      • adult 1 antibody
      • MGC133384 antibody
      • MYH1 antibody
      • MYH1_HUMAN antibody
      • MYHa antibody
      • MyHC 2x antibody
      • MyHC 2X/D antibody
      • MyHC IIx/d antibody
      • MyHC-2x antibody
      • MyHC-IIx/d antibody
      • MYHSA1 antibody
      • Myosin 1 antibody
      • Myosin heavy chain 1 antibody
      • Myosin heavy chain 2x antibody
      • Myosin heavy chain antibody
      • Myosin heavy chain IIx/d antibody
      • Myosin heavy chain, skeletal muscle, adult 1 antibody
      • Myosin, heavy chain 1, skeletal muscle, adult antibody
      • Myosin, heavy polypeptide 1, skeletal muscle, adult antibody
      • Myosin-1 antibody
      • skeletal muscle antibody
      see all

    Images

    • Western blot - Anti-MYH1 antibody (ab127539)
      Western blot - Anti-MYH1 antibody (ab127539)
      Anti-MYH1 antibody (ab127539) at 1/1000 dilution + Mouse heart whole cell lysate at 50 µg

      Predicted band size: 223 kDa



      5% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab127539? Please let us know so that we can cite the reference in this datasheet.

    ab127539 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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