Key features and details
- Rabbit polyclonal to MYH1
- Suitable for: WB
- Reacts with: Pig
- Isotype: IgG
Product nameAnti-MYH1 antibody
See all MYH1 primary antibodies
DescriptionRabbit polyclonal to MYH1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Pig
Predicted to work with: Human
Recombinant fragment (His-tag) corresponding to Human MYH1 aa 585-794. Expressed in E.coli. N-terminal tag.
YAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLALLFVGATGAEAEA GGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKT PGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAI PEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDEK LAQLITRTQA
Database link: P12882
- WB: Recombinant human MYH1 protein; Pig skeletal muscle and heart lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml.|
Sequence similaritiesContains 1 IQ domain.
Contains 1 myosin head-like domain.
DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Cellular localizationCytoplasm > myofibril. Thick filaments of the myofibrils.
- Information by UniProt
- adult 1 antibody
- MGC133384 antibody
- MYH1 antibody
Anti-MYH1 antibody (ab232968) at 2.5 µg/ml + Recombinant human MYH1 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
All lanes : Anti-MYH1 antibody (ab232968) at 2.5 µg/ml
Lane 1 : Pig skeletal muscle tissue lysate
Lane 2 : Pig heart tissue lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232968 has not yet been referenced specifically in any publications.