• Product name

    Anti-MYH2 antibody [EPR5280]
    See all MYH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5280] to MYH2
  • Host species

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MYH2 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Mouse muscle, Rat muscle and Human trachea lysates
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab124937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 220 kDa (predicted molecular weight: 223 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Muscle contraction. Required for cytoskeleton organization.
    • Involvement in disease

      Defects in MYH2 are the cause of inclusion body myopathy type 3 (IBM3) [MIM:605637]. Hereditary inclusion body myopathies constitute a group of neuromuscular disorders characterized by slowly progressive distal and proximal weakness and a typical muscle pathology including rimmed vacuoles and filamentous inclusions. IBM3 is a variant of hereditary inclusion body myopathies and is characterized by autosomal dominant myopathy with joint contracture, ophthalmoplegia and rimmed vacuoles. Morphological analysis of muscle biopsies from patients indicate that the type 2A fibers frequently were abnormal, whereas other fiber types appeared normal.
    • Sequence similarities

      Contains 1 IQ domain.
      Contains 1 myosin head-like domain.
    • Domain

      The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
      Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
    • Cellular localization

      Cytoplasm > myofibril. Thick filaments of the myofibrils.
    • Information by UniProt
    • Database links

    • Alternative names

      • adult 2 antibody
      • Fast 2a myosin heavy chain antibody
      • IBM3 antibody
      • Inclusion body myopathy 3, autosomal dominant antibody
      • MYH2 antibody
      • MYH2_HUMAN antibody
      • MYH2A antibody
      • MYHas8 antibody
      • MyHC IIa antibody
      • MyHC-2a antibody
      • MyHC-IIa antibody
      • MYHSA2 antibody
      • Myosin heavy chain 2 antibody
      • Myosin heavy chain 2a antibody
      • Myosin heavy chain antibody
      • Myosin heavy chain IIa antibody
      • Myosin heavy chain skeletal muscle adult 2 antibody
      • Myosin heavy polypeptide 2 skeletal muscle adult antibody
      • Myosin-2 antibody
      • MYPOP antibody
      • skeletal muscle antibody
      • Type IIA myosin heavy chain antibody
      see all


    • All lanes : Anti-MYH2 antibody [EPR5280] (ab124937) at 1/10000 dilution

      Lane 1 : Mouse muscle lysate
      Lane 2 : Rat muscle lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 223 kDa
      Observed band size: 220 kDa
      why is the actual band size different from the predicted?

    • Anti-MYH2 antibody [EPR5280] (ab124937) at 1/10000 dilution + Human trachea lysate at 10 µg

      HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 223 kDa
      Observed band size: 220 kDa why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:

    • Zeng X  et al. Acylated and unacylated ghrelin inhibit atrophy in myotubes co-cultured with colon carcinoma cells. Oncotarget 8:72872-72885 (2017). Read more (PubMed: 29069832) »
    See 1 Publication for this product

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