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RecombinantRabMAb

Recombinant Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

Submit a review Submit a question References (6)
Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)
  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)
  • Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)
  • Anti-Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10891(2)] to MYH6 + Slow Skeletal Myosin Heavy chain
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)]

  • Description

    Rabbit monoclonal [EPR10891(2)] to MYH6 + Slow Skeletal Myosin Heavy chain

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 78%,76%,76%,76%,72%homology with Myosin 2 1 3 8 4, respectively. Cross-reactivity with these proteins has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His tagged recombinant Human Myosin-7 and 6 protein, mouse and rat heart lysates. IHC: Human skeletal muscle

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500. Detects a band of approximately 220 kDa (predicted molecular weight: 224 kDa).
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/500. Detects a band of approximately 220 kDa (predicted molecular weight: 224 kDa).
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)
    Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)
    Lane 1 : Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967) at 1/1000 dilution
    Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/1000 dilution

    All lanes : His tagged recombinant Human Myosin-6 protein(aa 1193-1337)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated)

    Predicted band size: 224 kDa



    ab185967 could recognize both Myosin 6 and 7 protein

  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)
    Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)
    Lane 1 : Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967) at 1/1000 dilution
    Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/1000 dilution (His Tag)

    All lanes : His tagged recombinant Human Myosin-7 protein(aa 1191-1335)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 224 kDa



    ab185967 could recognize both Myosin 6 and 7 protein

  • Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)
    Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)
    All lanes : Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967) at 1/500 dilution

    Lane 1 : Human adult heart lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IR680 at 1/10000 dilution

    Predicted band size: 224 kDa



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab185967 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

    Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain with ab185967 at 1/2000 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)
    Anti-Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

References (6)

Publishing research using ab185967? Please let us know so that we can cite the reference in this datasheet.

ab185967 has been referenced in 6 publications.

  • Huang S  et al. P2X7 Receptor Deficiency Ameliorates STZ-induced Cardiac Damage and Remodeling Through PKCß and ERK. Front Cell Dev Biol 9:692028 (2021). PubMed: 34395424
  • Tang X  et al. miR-760 regulates skeletal muscle proliferation in rheumatoid arthritis by targeting Myo18b. Mol Med Rep 20:4843-4854 (2019). PubMed: 31661144
  • Prows DR  et al. Characterization of a mouse model of hypereosinophilia-associated heart disease. Am J Physiol Heart Circ Physiol 317:H405-H414 (2019). PubMed: 31199184
  • Luo W  et al. Inhibition of EGFR-STAT3 attenuates cardiomyopathy in streptozotocin-induced type 1 diabetes. J Endocrinol 242:199-210 (2019). PubMed: 31277074
  • Kankeu C  et al. Quantitative proteomics and systems analysis of cultured H9C2 cardiomyoblasts during differentiation over time supports a 'function follows form' model of differentiation. Mol Omics 14:181-196 (2018). PubMed: 29770421
  • Ju H  et al. MicroRNA-378 promotes myogenic differentiation by targeting BMP4. Mol Med Rep 13:2194-200 (2016). PubMed: 26782975

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