Key features and details
- Mouse monoclonal [3-48 G5C7] to MYH6
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-MYH6 antibody [3-48 G5C7]
DescriptionMouse monoclonal [3-48 G5C7] to MYH6
This antibody recognizes alpha and beta type MYH6 fragments (LMM) of cardiac myosin heavy chains. It also reacts with LMM fragments of cardiac myosin and beta heavy chain of slow human skeletal muscle. The antibody does not crossreact with human myosin light chains Specific for: human, rat and mouse; other species not tested but it is expected to also recognize and heavy chain from canine, bovine and rabbit cardiac tissues.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Dog, Pig
Full length native protein (purified) corresponding to Human heavy chain cardiac Myosin. Human ventricular myosin.
- WB: Human fetal, human adult, mouse and rat heart tissue lysates, human, mouse and rat skeletal muscle tissue lysates. IHC-P: Human, mouse and rat heart tissues.
General notesMolecular weight if IgG: according to SDS-PAGE under mercaptoethanol (reduced conditions) 153.8 kd. Heavy chain: 49.7 kd light chain: 27.2 kd. Molecular weight of F(ab')2: according to SDS-PAGE under mercaptoethanol (reduced conditions) 116 kd. Heavy chain: 30.8 kd light chain: 27.2 kd
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferLyophilized from PBS pH 7.4
Concentration information loading...
PurityProtein G purified
Purification notesLyophilized protein G purified in PBS pH7.4 (Full IgG).
Clone number3-48 G5C7
Light chain typekappa
Our Abpromise guarantee covers the use of ab207926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 223 kDa.|
|IHC-P||Use a concentration of 1 µg/ml.|
Involvement in diseaseAtrial septal defect 3
Cardiomyopathy, familial hypertrophic 14
Cardiomyopathy, dilated 1EE
Sick sinus syndrome 3
Sequence similaritiesContains 1 IQ domain.
Contains 1 myosin head-like domain.
DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Cellular localizationCytoplasm > myofibril. Thick filaments of the myofibrils.
- Information by UniProt
- cardiac muscle alpha isoform antibody
- MYH6 antibody
- MYH6_HUMAN antibody
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse heart tissue labeling MYH6 with ab207926 at 1 µg/ml.
All lanes : Anti-MYH6 antibody [3-48 G5C7] (ab207926) at 1/2000 dilution
Lane 1 : Human fetal heart tissue lysate at 20 µg
Lane 2 : Human fetal heart tissue lysate at 5 µg
Lane 3 : Human adult heart tissue lysate at 20 µg
Lane 4 : Mouse heart tissue lysate at 20 µg
Lane 5 : Rat heart tissue lysate at 20 µg
Lane 6 : Human skeletal muscle tissue lysate at 20 µg
Lane 7 : Mouse skeletal muscle tissue lysate at 20 µg
Lane 8 : Rat skeletal muscle tissue lysate at 20 µg
Lane 9 : Human liver tissue lysate at 20 µg
Predicted band size: 223 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human heart tissue labeling MYH6 with ab207926 at 1 µg/ml.
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat heart tissue labeling MYH6 with ab207926 at 1 µg/ml.
ab207926 has been referenced in 3 publications.
- Dal-Pra S et al. Demethylation of H3K27 Is Essential for the Induction of Direct Cardiac Reprogramming by miR Combo. Circ Res 120:1403-1413 (2017). PubMed: 28209718
- Liu Y et al. MicroRNA-26a protects against cardiac hypertrophy via inhibiting GATA4 in rat model and cultured cardiomyocytes. Mol Med Rep 14:2860-6 (2016). PubMed: 27485101
- Vogelpohl I et al. Transgenic overexpression of heart-specific adenine nucleotide translocase 1 positively affects contractile function in cardiomyocytes. Cell Physiol Biochem 27:121-8 (2011). PubMed: 21325829