Overview

  • Product name
    Anti-MYH6 antibody [BA-G5]
    See all MYH6 primary antibodies
  • Description
    Mouse monoclonal [BA-G5] to MYH6
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Pig
    Predicted to work with: Cow
  • Immunogen

    Cow skeletal muscle myosin

  • Positive control
    • This antibody gave a positive signal in the following Tissue Lysates: Mouse Skeletal Muscle, Rat Heart, Rat Skeletal Muscle
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab50967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IHC-P 1/100.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20139090

Target

Images

  • ab50967 staining MYH6 in murine HL-1 cells (derived from an atrial tumor) by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with 10% serum for 30 minutes at 21°C and then incubated with ab50967 at a 1/200 dilution for 16 hours at 4°C. The secondary used was a goat anti-mouse polyclonal conjugated to Alexa-Fluor 488, used at a 1/400 dilution. Counterstained with DAPI.

    See Abreview

  • Anti-MYH6 antibody [BA-G5] (ab50967) at 5 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 223 kDa
    Observed band size: 223 kDa

  • ab50967 staining MYH6 in mouse heart tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer pH 6, prior to blocking with sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C.  A HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • ab50967 at a 1/200 dilution staining MYH6 in murine heterozygote cardiomyocytes by Immunocytochemistry/ Immunofluorescence.

  • All lanes : Anti-MYH6 antibody [BA-G5] (ab50967) at 1 µg/ml

    Lane 1 : Heart (Rat) Tissue Lysate
    Lane 2 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 223 kDa
    Observed band size: 223 kDa
    Additional bands at: 48 kDa, 95 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • ab50967 staining MYH6 in Mouse whole embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in 5% Goat serum) for 16 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
See all 54 Publications for this product

Customer reviews and Q&As

11-13 of 13 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cardiac)
Specification
cardiac
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 05 2010

Application
Western blot
Sample
Human Tissue lysate - whole (heart atria)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
15 µg
Specification
heart atria
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Prof. Paul Townsend

Verified customer

Submitted Apr 21 2008

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
sequential peroxidase and protein block (prediluted) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Apr 03 2008

11-13 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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