Overview

  • Product name

  • Description

    Rabbit polyclonal to MYL12A
  • Host species

    Rabbit
  • Specificity

    ab131530 detects endogenous levels of total MYL12A.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MYL12A aa 16-20 conjugated to keyhole limpet haemocyanin. (A-T-S-N-V)
    Database link: NP_006462.1

  • Positive control

    • Methanol-fixed HeLa cells; K562 cell extracts.
  • General notes

    Previously labelled as MRCL3.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131530 was purified by affinity chromatography using epitope specific peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 20 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • Sequence similarities

    Contains 3 EF-hand domains.
  • Post-translational
    modifications

    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
  • Information by UniProt
  • Database links

  • Alternative names

    • ML12A_HUMAN antibody
    • MLC 2B antibody
    • MLC-2B antibody
    • MLCB antibody
    • MRCL3 antibody
    • MYL12A antibody
    • Myosin regulatory light chain 12A antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2, nonsarcomeric antibody
    • Myosin regulatory light chain MRCL3 antibody
    • Myosin regulatory light chain MRLC3 antibody
    • Myosin RLC antibody
    • Myosin, light polypeptide, regulatory, non sarcomeric (20kD) antibody
    • nonsarcomeric antibody
    • RLC antibody
    see all

Images

  • All lanes : Anti-MYL12A antibody (ab131530) at 1/500 dilution

    Lane 1 : K562 cell extract with blocking peptide
    Lane 2 : K562 cell extract

    Predicted band size: 20 kDa

  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling MYL12A with ab131530 at 1/100 dilution.

References

ab131530 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131530.
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