Overview

  • Product name

    Anti-MYL12A antibody [EP1456Y]
    See all MYL12A primary antibodies
  • Description

    Rabbit monoclonal [EP1456Y] to MYL12A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MYL12A (N terminal). The exact sequence is proprietary.

  • Positive control

    • 293T cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as MRCL3.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP1456Y
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 20 kDa.
ICC 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
    • Sequence similarities

      Contains 3 EF-hand domains.
    • Post-translational
      modifications

      Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
    • Information by UniProt
    • Database links

    • Alternative names

      • ML12A_HUMAN antibody
      • MLC 2B antibody
      • MLC-2B antibody
      • MLCB antibody
      • MRCL3 antibody
      • MYL12A antibody
      • Myosin regulatory light chain 12A antibody
      • Myosin regulatory light chain 2 antibody
      • Myosin regulatory light chain 2, nonsarcomeric antibody
      • Myosin regulatory light chain MRCL3 antibody
      • Myosin regulatory light chain MRLC3 antibody
      • Myosin RLC antibody
      • Myosin, light polypeptide, regulatory, non sarcomeric (20kD) antibody
      • nonsarcomeric antibody
      • RLC antibody
      see all

    Images

    • Anti-MYL12A antibody [EP1456Y] (ab81273) at 1/1000 dilution + 293T cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size: 20 kDa
      Observed band size: 20 kDa

    References

    ab81273 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab81273.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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