Recombinant
RabMAb

Recombinant Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] (ab126739)

Overview

  • Product name

    Anti-MYL12A (phospho T17 + S18) antibody [EPR1457]
    See all MYL12A primary antibodies
  • Description

    Rabbit monoclonal [EPR1457] to MYL12A (phospho T17 + S18)
  • Host species

    Rabbit
  • Specificity

    This antibody only detects MYL12A phosphorylated at Threonine 17 and Serine 18.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MYL12A (phospho T17 + S18).
    Database link: P19105

  • Positive control

    • NIH/3T3 cell lysate.
  • General notes

     

     

    Previously labelled as MRCL3.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR1457
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
    • Sequence similarities

      Contains 3 EF-hand domains.
    • Post-translational
      modifications

      Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
    • Information by UniProt
    • Database links

    • Alternative names

      • ML12A_HUMAN antibody
      • MLC 2B antibody
      • MLC-2B antibody
      • MLCB antibody
      • MRCL3 antibody
      • MYL12A antibody
      • Myosin regulatory light chain 12A antibody
      • Myosin regulatory light chain 2 antibody
      • Myosin regulatory light chain 2, nonsarcomeric antibody
      • Myosin regulatory light chain MRCL3 antibody
      • Myosin regulatory light chain MRLC3 antibody
      • Myosin RLC antibody
      • Myosin, light polypeptide, regulatory, non sarcomeric (20kD) antibody
      • nonsarcomeric antibody
      • RLC antibody
      see all

    Images

    • All lanes : Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] (ab126739) at 1/1000 dilution

      Lane 1 : NIH/3T3 cell
      lysate
      Lane 2 : NIH/3T3 cell
      lysate + Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 20 kDa
      Observed band size: 18 kDa
      why is the actual band size different from the predicted?

    References

    ab126739 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab126739.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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