Product nameAnti-MYL12A (phospho T17 + S18) antibody [EPR1457]
See all MYL12A primary antibodies
DescriptionRabbit monoclonal [EPR1457] to MYL12A (phospho T17 + S18)
SpecificityThis antibody only detects MYL12A phosphorylated at Threonine 17 and Serine 18.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human MYL12A (phospho T17 + S18).
Database link: P19105
- NIH/3T3 cell lysate.
Previously labelled as MRCL3.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).|
FunctionMyosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Sequence similaritiesContains 3 EF-hand domains.
modificationsPhosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
- Information by UniProt
- ML12A_HUMAN antibody
- MLC 2B antibody
- MLC-2B antibody
All lanes : Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] (ab126739) at 1/1000 dilution
Lane 1 : NIH/3T3 cell
Lane 2 : NIH/3T3 cell
lysate + Calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
ab126739 has not yet been referenced specifically in any publications.