Key features and details
- Rabbit polyclonal to MYL12B
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MYL12B antibody
See all MYL12B primary antibodies
DescriptionRabbit polyclonal to MYL12B
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Fusion protein corresponding to Human MYL12B aa 1-172. (Full length, BC004994).
MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFID KEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPE DVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDK KGNFNYIEFTRILKHGAKDKDD
Database link: O14950
- Mouse skeletal muscle, heart and bladder tissue lysates; Human breast cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/100.|
|WB||1/450 - 1/2000. Predicted molecular weight: 20 kDa.|
FunctionMyosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.
Tissue specificityUbiquitously expressed in various hematopoietic cells.
Sequence similaritiesContains 3 EF-hand domains.
modificationsPhosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.
- Information by UniProt
- ML12B_HUMAN antibody
- MLC 2A antibody
- MLC-2 antibody
All lanes : Anti-MYL12B antibody (ab197929) at 1/450 dilution
Lane 1 : Mouse skeletal muscle lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse bladder tissue lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 20 kDa
Exposure time: 20 seconds
Gel: 8% SDS-PAGE
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling MYL12B with ab197929 at 1/20 dilution.
ab197929 has not yet been referenced specifically in any publications.