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RecombinantRabMAb

Recombinant Anti-MYL12B antibody [EPR9331] (ab137063)

Reviews (1)Q&A (1)References (6)
Western blot - Anti-MYL12B antibody [EPR9331] (ab137063)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)
  • Immunocytochemistry/ Immunofluorescence - Anti-MYL12B antibody [EPR9331] (ab137063)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)
  • Anti-MYL12B antibody [EPR9331] (ab137063)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9331] to MYL12B
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-MYL12B antibody [EPR9331]
    See all MYL12B primary antibodies

  • Description

    Rabbit monoclonal [EPR9331] to MYL12B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, K562 and Saos 2 cell lysates; Human colon and Human kidney tissues; Jurkat cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9331

  • Isotype

    IgG

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (1)
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.

  • Tissue specificity

    Ubiquitously expressed in various hematopoietic cells.

  • Sequence similarities

    Contains 3 EF-hand domains.

  • Post-translational
    modifications

    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ML12B_HUMAN antibody
    • MLC 2A antibody
    • MLC-2 antibody
    • MLC-2a antibody
    • MLC-B antibody
    • MLC20 antibody
    • MRLC2 antibody
    • MYL12B antibody
    • MYLC2B antibody
    • Myosin light chain 12B regulatory antibody
    • Myosin regulatory light chain 12B antibody
    • myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2-B antibody
    • myosin regulatory light chain 2-B, smooth muscle isoform antibody
    • Myosin regulatory light chain 20 kDa antibody
    • Myosin regulatory light chain MRLC2 antibody
    • myosin, light chain 12B, regulatory antibody
    • OTTHUMP00000162244 antibody
    • OTTHUMP00000165806 antibody
    • OTTHUMP00000165807 antibody
    • OTTHUMP00000165808 antibody
    • SHUJUN-1 antibody
    • smooth muscle isoform antibody
    see all

Images

  • Western blot - Anti-MYL12B antibody [EPR9331] (ab137063)
    Western blot - Anti-MYL12B antibody [EPR9331] (ab137063)
    All lanes : Anti-MYL12B antibody [EPR9331] (ab137063) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Saos 2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 20 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling MYL12B with ab137063 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MYL12B antibody [EPR9331] (ab137063)
    Immunocytochemistry/ Immunofluorescence - Anti-MYL12B antibody [EPR9331] (ab137063)
    Immunofluorescent staining of Jurkat cells labelling MYL12B with ab137063 at 1/250 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling MYL12B with ab137063 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-MYL12B antibody [EPR9331] (ab137063)
    Anti-MYL12B antibody [EPR9331] (ab137063)

References (6)

Publishing research using ab137063? Please let us know so that we can cite the reference in this datasheet.

ab137063 has been referenced in 6 publications.

  • Xu J  et al. Therapeutic effects of CXCR4+ subpopulation of transgene-free induced cardiosphere-derived cells on experimental myocardial infarction. Cell Prolif 54:e13041 (2021). PubMed: 33942933
  • Varberg KM  et al. Transgelin induces dysfunction of fetal endothelial colony-forming cells from gestational diabetic pregnancies. Am J Physiol Cell Physiol 315:C502-C515 (2018). PubMed: 29949406
  • Yao J  et al. miR-133b regulates proliferation and apoptosis in high-glucose-induced human retinal endothelial cells by targeting ras homolog family member A. Int J Mol Med 42:839-850 (2018). PubMed: 29786744
  • Yin Y  et al. Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance. Exp Ther Med 14:2221-2227 (2017). PubMed: 28962146
  • Piskareva O  et al. The development of cisplatin resistance in neuroblastoma is accompanied by epithelial to mesenchymal transition in vitro. Cancer Lett N/A:N/A (2015). PubMed: 25960282
  • Wu F  et al. Identification of phosphorylated MYL12B as a potential plasma biomarker for septic acute kidney injury using a quantitative proteomic approach. Int J Clin Exp Pathol 8:14409-16 (2015). WB ; Mouse . PubMed: 26823757

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-MYL12B antibody [EPR9331]

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample
Xenopus laevis Cell (embryo)
Specification
embryo
Permeabilization
Yes - triton-X100
Fixative
Trichloroacetic Acid
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 25 2014

Question

I need to order antibodies for MYL7 ((ab92721) and MYL2 (ab137063). While going through the datasheets, I've seen IHC images of these antibodies but no reference is given for them. Can you provide us with the references for those images. Also, as we are regular customers of your products, can you provide us a small gift sample of these antibodies as our project is in infancy at present.

I look forward for your early response.

Read More
Answer

Thank you for contacting us with your questions.

The IHC images on the datasheets for ab92721 and ab137063 were produced in the Epitomics lab, and they weren't taken from publications so there are no references. Ab92721 was used in one publication-

http://www.ncbi.nlm.nih.gov/pubmed/22037191?dopt=Abstract

We only have full size vials of our antibodies, so we don't have samples to send out. However, we do have a promotion on rabbit monoclonal antibodies that is valid until December 16. For this promotion, if you purchase any primary antibody you can receive a free rabbit monoclonal. Since both of these antibodies are rabbit monoclonals (also called RabMabs), you could purchase one and then receive the other for free. If you'd like to take advantage of this offer, please include the code RABMAB-XBSMG with your order.

I hope that this information will be useful, but please let me know if you have further questions or need anything else and I'll be happy to help.

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