Recombinant
RabMAb

Recombinant Anti-MYL12B antibody [EPR9331] (ab137063)

Reviews (1)Q&A (1)References (4)

Overview

  • Product name

    Anti-MYL12B antibody [EPR9331]
    See all MYL12B primary antibodies

  • Description

    Rabbit monoclonal [EPR9331] to MYL12B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues at the C terminal of Human MYL12B (UniProt ID: O14950).

  • Positive control

    • Jurkat, K562 and Saos 2 cell lysates; Human colon and Human kidney tissues; Jurkat cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9331

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab137063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.

    • Tissue specificity

      Ubiquitously expressed in various hematopoietic cells.

    • Sequence similarities

      Contains 3 EF-hand domains.

    • Post-translational
      modifications

      Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ML12B_HUMAN antibody
      • MLC 2A antibody
      • MLC-2 antibody
      • MLC-2a antibody
      • MLC-B antibody
      • MLC20 antibody
      • MRLC2 antibody
      • MYL12B antibody
      • MYLC2B antibody
      • Myosin light chain 12B regulatory antibody
      • Myosin regulatory light chain 12B antibody
      • myosin regulatory light chain 2 antibody
      • Myosin regulatory light chain 2-B antibody
      • myosin regulatory light chain 2-B, smooth muscle isoform antibody
      • Myosin regulatory light chain 20 kDa antibody
      • Myosin regulatory light chain MRLC2 antibody
      • myosin, light chain 12B, regulatory antibody
      • OTTHUMP00000162244 antibody
      • OTTHUMP00000165806 antibody
      • OTTHUMP00000165807 antibody
      • OTTHUMP00000165808 antibody
      • SHUJUN-1 antibody
      • smooth muscle isoform antibody
      see all

    Images

    • Western blot - Anti-MYL12B antibody [EPR9331] (ab137063)
      Western blot - Anti-MYL12B antibody [EPR9331] (ab137063)
      All lanes : Anti-MYL12B antibody [EPR9331] (ab137063) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Saos 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 20 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling MYL12B with ab137063 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-MYL12B antibody [EPR9331] (ab137063)
      Immunocytochemistry/ Immunofluorescence - Anti-MYL12B antibody [EPR9331] (ab137063)
      Immunofluorescent staining of Jurkat cells labelling MYL12B with ab137063 at 1/250 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling MYL12B with ab137063 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Yao J  et al. miR-133b regulates proliferation and apoptosis in high-glucose-induced human retinal endothelial cells by targeting ras homolog family member A. Int J Mol Med 42:839-850 (2018). Read more (PubMed: 29786744) »
    • Yin Y  et al. Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance. Exp Ther Med 14:2221-2227 (2017). Read more (PubMed: 28962146) »
    See all 4 Publications for this product

    Publishing research using ab137063? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MYL12B antibody [EPR9331]

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Sample
    Xenopus laevis Cell (embryo)
    Specification
    embryo
    Permeabilization
    Yes - triton-X100
    Fixative
    Trichloroacetic Acid
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 25 2014

    Question

    I need to order antibodies for MYL7 ((ab92721) and MYL2 (ab137063). While going through the datasheets, I've seen IHC images of these antibodies but no reference is given for them. Can you provide us with the references for those images. Also, as we are regular customers of your products, can you provide us a small gift sample of these antibodies as our project is in infancy at present.

    I look forward for your early response.

    Read More
    Answer

    Thank you for contacting us with your questions.

    The IHC images on the datasheets for ab92721 and ab137063 were produced in the Epitomics lab, and they weren't taken from publications so there are no references. Ab92721 was used in one publication-

    http://www.ncbi.nlm.nih.gov/pubmed/22037191?dopt=Abstract

    We only have full size vials of our antibodies, so we don't have samples to send out. However, we do have a promotion on rabbit monoclonal antibodies that is valid until December 16. For this promotion, if you purchase any primary antibody you can receive a free rabbit monoclonal. Since both of these antibodies are rabbit monoclonals (also called RabMabs), you could purchase one and then receive the other for free. If you'd like to take advantage of this offer, please include the code RABMAB-XBSMG with your order.

    I hope that this information will be useful, but please let me know if you have further questions or need anything else and I'll be happy to help.

    Read More

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