Recombinant Anti-MYL12B antibody [EPR9331] (ab137063)
![Western blot - Anti-MYL12B antibody [EPR9331] (ab137063)](https://www.abcam.com/ps/products/137/ab137063/Images/ab137063-151589-anti-myl12b-antibody-epr9331-western-blot.jpg "Western blot - Anti-MYL12B antibody [EPR9331] (ab137063)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9331] to MYL12B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-MYL12B antibody [EPR9331]
See all MYL12B primary antibodies -
Description
Rabbit monoclonal [EPR9331] to MYL12B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to residues at the C terminal of Human MYL12B (UniProt ID: O14950).
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Positive control
- Jurkat, K562 and Saos 2 cell lysates; Human colon and Human kidney tissues; Jurkat cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9331 -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF | (1) |
1/250 - 1/500.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/250 - 1/500. |
Application notes
Is unsuitable for Flow Cyt.
Target
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Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion. -
Tissue specificity
Ubiquitously expressed in various hematopoietic cells. -
Sequence similarities
Contains 3 EF-hand domains. -
Post-translational
modificationsPhosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment. - Information by UniProt
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Database links
- Entrez Gene: 103910 Human
- Entrez Gene: 67938 Mouse
- Entrez Gene: 50685 Rat
- Omim: 609211 Human
- SwissProt: O14950 Human
- SwissProt: Q3THE2 Mouse
- SwissProt: P18666 Rat
- Unigene: 190086 Human
see all -
Alternative names
- ML12B_HUMAN antibody
- MLC 2A antibody
- MLC-2 antibody
see all
Images
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Western blot - Anti-MYL12B antibody [EPR9331] (ab137063)All lanes : Anti-MYL12B antibody [EPR9331] (ab137063) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Saos 2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 20 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)](https://www.abcam.com/ps/products/137/ab137063/Images/ab137063-151587-anti-myl12b-antibody-epr9331-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling MYL12B with ab137063 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-MYL12B antibody [EPR9331] (ab137063)](https://www.abcam.com/ps/products/137/ab137063/Images/ab137063-151588-anti-myl12b-antibody-epr9331-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MYL12B antibody [EPR9331] (ab137063)Immunofluorescent staining of Jurkat cells labelling MYL12B with ab137063 at 1/250 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)](https://www.abcam.com/ps/products/137/ab137063/Images/ab137063-151586-anti-myl12b-antibody-epr9331-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (ab137063)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling MYL12B with ab137063 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-MYL12B antibody [EPR9331] (ab137063)](https://www.abcam.com/ps/products/137/ab137063/Images/ab137063-5-benefits-of-recombinant-antibodies.png)
Anti-MYL12B antibody [EPR9331] (ab137063)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab137063 has been referenced in 4 publications.
- Yao J et al. miR-133b regulates proliferation and apoptosis in high-glucose-induced human retinal endothelial cells by targeting ras homolog family member A. Int J Mol Med 42:839-850 (2018). PubMed: 29786744
- Yin Y et al. Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance. Exp Ther Med 14:2221-2227 (2017). PubMed: 28962146
- Piskareva O et al. The development of cisplatin resistance in neuroblastoma is accompanied by epithelial to mesenchymal transition in vitro. Cancer Lett N/A:N/A (2015). PubMed: 25960282
- Wu F et al. Identification of phosphorylated MYL12B as a potential plasma biomarker for septic acute kidney injury using a quantitative proteomic approach. Int J Clin Exp Pathol 8:14409-16 (2015). WB ; Mouse . PubMed: 26823757