Recombinant Anti-MYL12B antibody [EPR9331] (ab137063)


  • Product name

    Anti-MYL12B antibody [EPR9331]
    See all MYL12B primary antibodies
  • Description

    Rabbit monoclonal [EPR9331] to MYL12B
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues at the C terminal of Human MYL12B (UniProt ID: O14950).

  • Positive control

    • Jurkat, K562 and Saos 2 cell lysates; Human colon and Human kidney tissues; Jurkat cells
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

  • Clone number

  • Isotype



Our Abpromise guarantee covers the use of ab137063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.
    • Tissue specificity

      Ubiquitously expressed in various hematopoietic cells.
    • Sequence similarities

      Contains 3 EF-hand domains.
    • Post-translational

      Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.
    • Information by UniProt
    • Database links

    • Alternative names

      • ML12B_HUMAN antibody
      • MLC 2A antibody
      • MLC-2 antibody
      • MLC-2a antibody
      • MLC-B antibody
      • MLC20 antibody
      • MRLC2 antibody
      • MYL12B antibody
      • MYLC2B antibody
      • Myosin light chain 12B regulatory antibody
      • Myosin regulatory light chain 12B antibody
      • myosin regulatory light chain 2 antibody
      • Myosin regulatory light chain 2-B antibody
      • myosin regulatory light chain 2-B, smooth muscle isoform antibody
      • Myosin regulatory light chain 20 kDa antibody
      • Myosin regulatory light chain MRLC2 antibody
      • myosin, light chain 12B, regulatory antibody
      • OTTHUMP00000162244 antibody
      • OTTHUMP00000165806 antibody
      • OTTHUMP00000165807 antibody
      • OTTHUMP00000165808 antibody
      • SHUJUN-1 antibody
      • smooth muscle isoform antibody
      see all


    • All lanes : Anti-MYL12B antibody [EPR9331] (ab137063) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Saos 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 20 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling MYL12B with ab137063 at 1/100 dilution.
    • Immunofluorescent staining of Jurkat cells labelling MYL12B with ab137063 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling MYL12B with ab137063 at 1/100 dilution.


    This product has been referenced in:

    • Yao J  et al. miR-133b regulates proliferation and apoptosis in high-glucose-induced human retinal endothelial cells by targeting ras homolog family member A. Int J Mol Med 42:839-850 (2018). Read more (PubMed: 29786744) »
    • Yin Y  et al. Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance. Exp Ther Med 14:2221-2227 (2017). Read more (PubMed: 28962146) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Xenopus laevis Cell (embryo)
    Yes - triton-X100
    Trichloroacetic Acid

    Abcam user community

    Verified customer

    Submitted Jun 25 2014


    Thank you for contacting us with your questions.

    The IHC images on the datasheets for ab92721 and ab137063 were produced in the Epitomics lab, and they weren't taken from publications so there are no references. Ab92721 was used in one publication-

    We only have full size vials of our antibodies, so we don't have samples to send out. However, we do have a promotion on rabbit monoclonal antibodies that is valid until December 16. For this promotion, if you purchase any primary antibody you can receive a free rabbit monoclonal. Since both of these antibodies are rabbit monoclonals (also called RabMabs), you could purchase one and then receive the other for free. If you'd like to take advantage of this offer, please include the code RABMAB-XBSMG with your order.

    I hope that this information will be useful, but please let me know if you have further questions or need anything else and I'll be happy to help.

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