Key features and details
- Mouse polyclonal to MYL7
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MYL7 antibody
See all MYL7 primary antibodies
DescriptionMouse polyclonal to MYL7
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
MYL7 (1 a.a. ~ 175 a.a) full-length human protein.
- MYL7 transfected 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).|
Tissue specificityPredominantly expressed in adult atrial muscle.
Sequence similaritiesContains 3 EF-hand domains.
- Information by UniProt
- zgc:92755 antibody
- atrial isoform antibody
- cmlc2 antibody
All lanes : Anti-MYL7 antibody (ab68086) at 1/500 dilution
Lane 1 : MYL7 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
IF/ICC image of MYL7 antibody (ab68086) staining hES cell derived cardiomyocytes (red). The cells were fixed in paraformaldehyde and blocked in 4% goat serum for 1 hour at 25°C. The MYL7 antibody was used at a concentration of 1:100, for 16 hours at 4°C.
ab68086 has been referenced in 3 publications.
- Crombie DE et al. Friedreich's ataxia induced pluripotent stem cell-derived cardiomyocytes display electrophysiological abnormalities and calcium handling deficiency. Aging (Albany NY) 9:1440-1452 (2017). ICC/IF ; Human . PubMed: 28562313
- Ang YS et al. Disease Model of GATA4 Mutation Reveals Transcription Factor Cooperativity in Human Cardiogenesis. Cell 167:1734-1749.e22 (2016). PubMed: 27984724
- Ifkovits JL et al. Inhibition of TGFß signaling increases direct conversion of fibroblasts to induced cardiomyocytes. PLoS One 9:e89678 (2014). ICC ; Mouse . PubMed: 24586958