Overview

  • Product name

  • Description

    Mouse polyclonal to MYL7
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    MYL7 (1 a.a. ~ 175 a.a) full-length human protein.

  • Positive control

    • MYL7 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

Target

  • Tissue specificity

    Predominantly expressed in adult atrial muscle.
  • Sequence similarities

    Contains 3 EF-hand domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • zgc:92755 antibody
    • atrial isoform antibody
    • cmlc2 antibody
    • MGC92755 antibody
    • MLC-2a antibody
    • MLC2a antibody
    • MLCK, cardiac antibody
    • MLRA_HUMAN antibody
    • MYL2A antibody
    • MYL7 antibody
    • Mylc2a antibody
    • Myosin light chain 2a antibody
    • Myosin light chain 7 regulatory antibody
    • Myosin light polypeptide 7 regulatory antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 atrial isoform antibody
    • Myosin regulatory light chain 7 antibody
    see all

Images

  • All lanes : Anti-MYL7 antibody (ab68086) at 1/500 dilution

    Lane 1 : MYL7 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • IF/ICC image of MYL7 antibody (ab68086) staining hES cell derived cardiomyocytes (red). The cells were fixed in paraformaldehyde and blocked in 4% goat serum for 1 hour at 25°C. The MYL7 antibody was used at a concentration of 1:100, for 16 hours at 4°C.

    See Abreview

References

This product has been referenced in:

  • Crombie DE  et al. Friedreich's ataxia induced pluripotent stem cell-derived cardiomyocytes display electrophysiological abnormalities and calcium handling deficiency. Aging (Albany NY) 9:1440-1452 (2017). ICC/IF ; Human . Read more (PubMed: 28562313) »
  • Ang YS  et al. Disease Model of GATA4 Mutation Reveals Transcription Factor Cooperativity in Human Cardiogenesis. Cell 167:1734-1749.e22 (2016). Read more (PubMed: 27984724) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hESC-derived cardiomyocyte)
Specification
hESC-derived cardiomyocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 18 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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