Overview

  • Product name

  • Description

    Rabbit polyclonal to MYL9
  • Host species

    Rabbit
  • Specificity

    ab183069 detects endogenous level of total MYL9 protein.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Pig, Orangutan
  • Immunogen

    Synthetic peptide within Human MYL9 aa 2-14. The exact sequence is proprietary. Genbank NP_006088
    Sequence:

    SSKRAKAKTTKKR


    Database link: P24844

  • Positive control

    • Jurkat and 231 cell lysates; Human liver cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 49% PBS

    (PBS is without Mg2+ and Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 20 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • Tissue specificity

    Smooth muscle tissues and in some, but not all, nonmuscle cells.
  • Sequence similarities

    Contains 3 EF-hand domains.
  • Post-translational
    modifications

    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
  • Information by UniProt
  • Database links

  • Alternative names

    • 20 kDa myosin light chain antibody
    • Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody
    • LC20 antibody
    • MGC3505 antibody
    • MLC 2 antibody
    • MLC-2C antibody
    • MLC2 antibody
    • MLY 9 antibody
    • MRLC1 antibody
    • MYL9 antibody
    • MYL9_HUMAN antibody
    • Myosin light chain 9 regulatory antibody
    • Myosin light polypeptide 9 regulatory antibody
    • myosin regulatory light chain 1 antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 smooth muscle isoform antibody
    • Myosin regulatory light chain 9 antibody
    • Myosin regulatory light chain MRLC1 antibody
    • Myosin regulatory light polypeptide 9 antibody
    • Myosin RLC antibody
    • Myosin vascular smooth muscle light chain 2 antibody
    • MYRL2 antibody
    • OTTHUMP00000030857 antibody
    • smooth muscle isoform antibody
    see all

Images

  • All lanes : Anti-MYL9 antibody (ab183069) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : MM231 cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 20 kDa


    Exposure time: 30 seconds
  • Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling MYL9 with ab183069 at a 1/80 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)

References

ab183069 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183069.
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