Recombinant Anti-MYL9 antibody [EPR13012(2)] (ab191393)
![Western blot - Anti-MYL9 antibody [EPR13012(2)] (ab191393)](https://www.abcam.com/ps/products/191/ab191393/Images/ab191393-389661-anti-myl9-antibody-epr130122-western-blot--human-lysate.jpg "Western blot - Anti-MYL9 antibody [EPR13012(2)] (ab191393)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13012(2)] to MYL9
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MYL9 antibody [EPR13012(2)]
See all MYL9 primary antibodies -
Description
Rabbit monoclonal [EPR13012(2)] to MYL9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Hu colon, C6, PC12 and NIH 3T3 cell lysates; Human stomach, colon cancer and uterus lysates; Rat stomach lysate. IHC-P: Human and rat colon tissues. IP: Human stomach lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13012(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
|
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| IHC-P |
1/350 - 1/700. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/40.
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| Notes |
|---|
|
WB
1/1000 - 1/2000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
|
IHC-P
1/350 - 1/700. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
1/40. |
Target
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Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. -
Tissue specificity
Smooth muscle tissues and in some, but not all, nonmuscle cells. -
Sequence similarities
Contains 3 EF-hand domains. -
Post-translational
modificationsPhosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. - Information by UniProt
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Database links
- Entrez Gene: 10398 Human
- Entrez Gene: 98932 Mouse
- Entrez Gene: 296313 Rat
- Omim: 609905 Human
- SwissProt: P24844 Human
- SwissProt: Q9CQ19 Mouse
- SwissProt: Q64122 Rat
- Unigene: 504687 Human
see all -
Alternative names
- 20 kDa myosin light chain antibody
- Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody
- LC20 antibody
see all
Images
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Western blot - Anti-MYL9 antibody [EPR13012(2)] (ab191393)All lanes : Anti-MYL9 antibody [EPR13012(2)] (ab191393) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MYL9 knockout HeLa cell lysate
Lane 3 : Human colon cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDaLanes 1-4: Merged signal (red and green). Green - ab191393 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.
ab191393 Anti-MYL9 antibody [EPR13012(2)] was shown to specifically react with MYL9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266036 (knockout cell lysate ab256999) was used. Wild-type and MYL9 knockout samples were subjected to SDS-PAGE. ab191393 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 [EPR13012(2)] antibody (ab191393)](https://www.abcam.com/ps/products/191/ab191393/Images/ab191393-228691-ab1913934.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 [EPR13012(2)] antibody (ab191393)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MYL9 with ab191393 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)](https://www.abcam.com/ps/products/191/ab191393/Images/ab191393-228695-ab1913931.jpg)
Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)All lanes : Anti-MYL9 antibody [EPR13012(2)] (ab191393) at 1/1000 dilution
Lane 1 : Human stomach lysate
Lane 2 : Human colon cancer lysate
Lane 3 : Human uterus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa -
![Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)](https://www.abcam.com/ps/products/191/ab191393/Images/ab191393-228694-ab1913932.jpg)
Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)All lanes : Anti-MYL9 antibody [EPR13012(2)] (ab191393) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa -
![Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)](https://www.abcam.com/ps/products/191/ab191393/Images/ab191393-228692-ab1913933.jpg)
Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)Anti-MYL9 antibody [EPR13012(2)] (ab191393) at 1/5000 dilution + rat stomach lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 [EPR13012(2)] antibody (ab191393)](https://www.abcam.com/ps/products/191/ab191393/Images/ab191393-228690-ab1913935.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 [EPR13012(2)] antibody (ab191393)Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MYL9 with ab191393 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-MYL9 [EPR13012(2)] antibody (ab191393)](https://www.abcam.com/ps/products/191/ab191393/Images/ab191393-228689-ab191393.jpg)
Immunoprecipitation - Anti-MYL9 [EPR13012(2)] antibody (ab191393)Western blot analysis of immunoprecipitation pellet from Human stomach lysate immunoprecipitated using ab191393 at 1/40 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Anti-MYL9 antibody [EPR13012(2)] (ab191393)](https://www.abcam.com/ps/products/191/ab191393/Images/ab191393-8-benefits-of-recombinant-antibodies.png)
Anti-MYL9 antibody [EPR13012(2)] (ab191393)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab191393 has been referenced in 4 publications.
- Wang J et al. HPV E7 affects the function of cervical cancer cells via the TAL1/lnc-EBIC/KLHDC7B axis. Oncol Rep 45:N/A (2021). PubMed: 33760214
- Sun J et al. Distinct Roles of Smooth Muscle and Non-muscle Myosin Light Chain-Mediated Smooth Muscle Contraction. Front Physiol 11:593966 (2020). PubMed: 33424621
- Hu X et al. Effects of ß-hydroxybutyric acid and ghrelin on the motility and inflammation of gastric antral smooth muscle cells involving the regulation of growth hormone secretagogue receptor. Mol Med Rep 20:5050-5058 (2019). PubMed: 31638214
- Wang JH et al. Expression and prognostic significance of MYL9 in esophageal squamous cell carcinoma. PLoS One 12:e0175280 (2017). IHC-P ; Human . PubMed: 28388691