Recombinant
RabMAb

Recombinant Anti-MYL9 antibody [EPR13012(2)] - BSA and Azide free (ab236126)

Overview

  • Product name

    Anti-MYL9 antibody [EPR13012(2)] - BSA and Azide free
    See all MYL9 primary antibodies
  • Description

    Rabbit monoclonal [EPR13012(2)] to MYL9 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MYL9 aa 50-150. The exact sequence is proprietary.
    Database link: P24844

  • Positive control

    • IHC-P: Human colon tissue.
  • General notes

    Ab236126 is the carrier-free version of ab191393. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236126 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13012(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Target

  • Function

    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • Tissue specificity

    Smooth muscle tissues and in some, but not all, nonmuscle cells.
  • Sequence similarities

    Contains 3 EF-hand domains.
  • Post-translational
    modifications

    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
  • Information by UniProt
  • Database links

  • Alternative names

    • 20 kDa myosin light chain antibody
    • Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody
    • LC20 antibody
    • MGC3505 antibody
    • MLC 2 antibody
    • MLC-2C antibody
    • MLC2 antibody
    • MLY 9 antibody
    • MRLC1 antibody
    • MYL9 antibody
    • MYL9_HUMAN antibody
    • Myosin light chain 9 regulatory antibody
    • Myosin light polypeptide 9 regulatory antibody
    • myosin regulatory light chain 1 antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 smooth muscle isoform antibody
    • Myosin regulatory light chain 9 antibody
    • Myosin regulatory light chain MRLC1 antibody
    • Myosin regulatory light polypeptide 9 antibody
    • Myosin RLC antibody
    • Myosin vascular smooth muscle light chain 2 antibody
    • MYRL2 antibody
    • OTTHUMP00000030857 antibody
    • smooth muscle isoform antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MYL9 with ab191393 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191393).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot analysis of immunoprecipitation pellet from Human stomachlysate immunoprecipitated using ab191393 at 1/40 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191393).

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MYL9 with ab191393 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191393).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab236126 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236126.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up