Overview

  • Product name

    Anti-Mylc2b (phospho S18) antibody
  • Description

    Rabbit polyclonal to Mylc2b (phospho S18)
  • Host species

    Rabbit
  • Specificity

    ab63479 detects Mylc2b only when phosphorylated at serine 18 (Human: Ser18; Mouse: Ser20; Rat: Ser19).
  • Tested applications

    Suitable for: IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide (Human) derived from Mylc2b from around the phosphorylation site of serine 18 (A-T-SP-N-V).

  • Positive control

    • Human brain tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab63479 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/20000.

Target

  • Relevance

    Mylc2b (Myosin regulatory light chain 2 B, smooth muscle isoform) plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity.
  • Database links

  • Alternative names

    • Myosin light chain regulatory B antibody
    • Myosin regulatory light chain 2 B, smooth muscle isoform antibody
    • RLC B antibody

Images

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using Mylc2b (phospho S18) antibody (ab63479) at 1/50 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.

References

This product has been referenced in:

  • Wu F  et al. Identification of phosphorylated MYL12B as a potential plasma biomarker for septic acute kidney injury using a quantitative proteomic approach. Int J Clin Exp Pathol 8:14409-16 (2015). Read more (PubMed: 26823757) »
See 1 Publication for this product

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