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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH
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Myo-D positive control ChIP primer pair (ab269261)

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ChIP - Myo-D positive control ChIP primer pair (ab269261)
  • ChIP - Myo-D positive control ChIP primer pair (ab269261)
  • ChIP - Myo-D positive control ChIP primer pair (ab269261)

Key features and details

  • Myo-D positive control ChIP primer pair
  • Suitable for: ChIP

Overview

  • Product name

    Myo-D positive control ChIP primer pair
    See all MyoD1 primary antibodies
  • Description

    Myo-D positive control ChIP primer pair
  • Tested applications

    Suitable for: ChIPmore details
  • General notes

    Positive control ChIP-qPCR 5' and 3' primers for Myo-D gene. Use with SYBR green.

    We recommend these primers as a positive control (based on Abcam's testing) for the histone marks below. They may also be useful for other histone marks.

    Suitable positive control for:
    - Histone H3 tri methyl K27
    - unmodified Histone H3
    - Histone H3 mono methyl K4
    - unmodified Histone H2B
    - unmodified Histone H4
    - Histone H3 mono methyl K9
    - Histone H4 mono methyl K20
    - unmodified Histone H2A 

    500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.

    Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25µl.

    Please contact us after purchase if you require the sequence of the oligos.

Properties

  • Form

    Lyophilized
  • Storage instructions

    Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Muscle

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP
Use at an assay dependent concentration.
Notes
ChIP
Use at an assay dependent concentration.

Target

  • Function

    Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications

    Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
    Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P15172 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • bHLHc1 antibody
    • Class C basic helix-loop-helix protein 1 antibody
    • MYF 3 antibody
    • Myf-3 antibody
    • MYF3 antibody
    • Myoblast determination protein 1 antibody
    • Myod 1 antibody
    • MYOD antibody
    • MYOD1 antibody
    • MYOD1_HUMAN antibody
    • Myogenic differentiation 1 antibody
    • Myogenic factor 3 antibody
    • Myogenic factor MYF 3 antibody
    • Myogenin D1 antibody
    • PUM antibody
    see all

Images

  • ChIP - Myo-D positive control ChIP primer pair (ab269261)
    ChIP - Myo-D positive control ChIP primer pair (ab269261)

    Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 5 µg of ab6002 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • ChIP - Myo-D positive control ChIP primer pair (ab269261)
    ChIP - Myo-D positive control ChIP primer pair (ab269261)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab176880 (red), and 20µl of Protein A/G sepharose beads. Rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • ChIP - Myo-D positive control ChIP primer pair (ab269261)
    ChIP - Myo-D positive control ChIP primer pair (ab269261)

    Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with 0.75% formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab176877 (blue), and 20μl of Anti-rabbit IgG agarose beads. Rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH and ALDOA (active) and MYO-D (inactive) promoters and over the γ-Actin gene (active). Schematic diagram of the γ-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab269261? Please let us know so that we can cite the reference in this datasheet.

    ab269261 has not yet been referenced specifically in any publications.

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