Key features and details
- Rabbit polyclonal to MYO18A
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MYO18A antibody
See all MYO18A primary antibodies
DescriptionRabbit polyclonal to MYO18A
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 300-350 of human MYO18A (NP_510880.2).
- RIPA whole cell lysate from HeLa cells.
ab81689 has not performed satisfactorily when used for WB of MYO18A in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MYO18A.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab81689 was affinity purified using an epitope specific to MYO18A immobilized on solid support.
Primary antibody notesab81689 has not performed satisfactorily when used for WB of MYO18A in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MYO18A.
Our Abpromise guarantee covers the use of ab81689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceMyo18A/Myosin XVIIIA is an unconventional myosin that contains a lysine and glutamine (KE) rich domain and a PDZ domain. The KE and PDZ domains appear to direct subcellular localization of the Myo18A variants. It is a dimeric actin binding protein that may play a role in the stabilization and organization of the actin cytoskeleton. Recently a cell surface form of Myo18A has been identified as the receptor for surfactant protein A, a lipid associated lung collectin that mediates innate and adaptive immune responses in the lungs.
Cellular localizationIsoform 1: Endoplasmic reticulum Golgi intermediate compartment. Cytoplasm, cytoskeleton. Note: Colocalizes with actin. Isoform 2: Cytoplasm. Note: Lacks the PDZ domain. Diffusely localized in the cytoplasm.
- MAJN antibody
- Molecule associated with JAK3 N terminus antibody
- Myosin 18A antibody
Detection of human MYO18A by Western Blot of Immunoprecipitates.
Samples: RIPA whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
ab81689 used for IP at 3 µg/mg lysate (lane 1).
MYO18A was also immunoprecipitated using anti MYO18A (ab81690), which recognizes a downstream epitope (lane 2).
Lane 3 represents the control IgG.
For blotting immunoprecipitates, ab81689 was used at 1 µg/ml.
Detection: by Chemiluminescence with an exposure time of 1 second.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab81689 has not yet been referenced specifically in any publications.